Wada, Masaru; Narita, Kotomi & Yokota, Atsushi. Alanine production in an H+-ATPase- and lactate dehydrogenase-defective mutant of Escherichia coli expressing alanine dehydrogenase. Applied Microbiology and Biotechnology. 2007, 76(4), 819-825.

Strains

• Escherichia coli W1485lip2
• Escherichia coli TBLA-1
• Escherichia coli TBLA-1ΔldhA
• Escherichia coli W1485lip2/pUCADH
• Escherichia coli W1485lip2/pSTVADH
• Escherichia coli W1485lip2/pHSGADH
• Escherichia coli TBLA-1/pUCADH
• Escherichia coli TBLA-1/pSTVADH
• Escherichia coli TBLA-1/pHSGADH
• Escherichia coli TBLA-1ΔldhA/pSTVADH

Plasmids

• pUCADH
• pSTVADH
• pHSG576
• pHSGADH

Metabolite production

• pyruvate
  Titer
  • 30.0 g/L (Escherichia coli TBLA-1 in Batch)
  • 25.0 g/L (Escherichia coli W1485lip2 in Batch)
  • 16.0 g/L (Escherichia coli TBLA-1ΔldhA/pSTVADH in Batch)
  • 13.8 g/L (Escherichia coli TBLA-1/pSTVADH in Batch)
  • 17.3 g/L (Escherichia coli W1485lip2/pSTVADH in Batch)
• L-alanine
  Titer
  • 1.0 g/L (Escherichia coli TBLA-1/pUCADH in Flask)
  • 3.6 g/L (Escherichia coli TBLA-1/pSTVADH in Flask)
  • 3.0 g/L (Escherichia coli TBLA-1/pHSGADH in Flask)
  • 1.3 g/L (Escherichia coli W1485lip2/pUCADH in Flask)
  • 2.3 g/L (Escherichia coli W1485lip2/pSTVADH in Flask)
  • 0.85 g/L (Escherichia coli W1485lip2/pHSGADH in Flask)
  • 20.0 g/L (Escherichia coli TBLA-1ΔldhA/pSTVADH in Batch)
  • 14.7 g/L (Escherichia coli TBLA-1/pSTVADH in Batch)
  • 10.9 g/L (Escherichia coli W1485lip2/pSTVADH in Batch)
  Substrate yield
  • 0.408 mol/mol of substrate (Escherichia coli TBLA-1ΔldhA/pSTVADH in Batch)
  • 0.3 mol/mol of substrate (Escherichia coli TBLA-1/pSTVADH in Batch)
  • 0.223 mol/mol of substrate (Escherichia coli W1485lip2/pSTVADH in Batch)