Li, Jinyang; Shen, Jiawen; Ye, Wuyue; Tang, Xinyan; Wang, Zhiyu; Geng, Muyun; Liu, Yunye; Chen, Xianzhong & Zhou, Li. Dynamically metabolic engineering overflow metabolism for efficient production of l-alanine in Escherichia coli. Bioresource Technology. 2025, 428, 132446.

Strains

• Escherichia coli B0016-060BC-sacB-tetA
• Escherichia coli B0016-070BC
• Escherichia coli B0016-070BC-sacB-tetA
• Escherichia coli B0016-080BC
• Escherichia coli B0016-060B
• Escherichia coli B0016-090BC
• Escherichia coli B0016-060BC

Metabolite production

• L-alanine
  Titer
  • 195.2 g/L (Escherichia coli B0016-090BC in Batch)
  • 167.7 g/L (Escherichia coli B0016-060BC in Fed-Batch)
  • 182.6 g/L (Escherichia coli B0016-080BC in Fed-Batch)
  • 120.8 g/L (Escherichia coli B0016-060BC in Batch)
  Substrate yield
  • 0.886 g/g of sustrate (Escherichia coli B0016-090BC in Batch)
  • 0.863 g/g of sustrate (Escherichia coli B0016-080BC in Fed-Batch)
  Volumetric productivity
  • 3.07 g/L/h (Escherichia coli B0016-090BC in Batch)
  • 2.96 g/L/h (Escherichia coli B0016-060BC in Fed-Batch)
  • 3.07 g/L/h (Escherichia coli B0016-080BC in Fed-Batch)