Pengfei Gu, Qianqian Ma, Shuo Zhao, Qiang Li & Juan Gao (2023). Alanine dehydrogenases from four different microorganisms: characterization and their application in L-alanine production. Biotechnology for Biofuels and Bioproducts.

Strains

• Escherichia coli M-1
• Escherichia coli M-2
• Escherichia coli M-3
• Escherichia coli M-4
• Escherichia coli M-6
• Escherichia coli M-5
• Escherichia coli M-7
• Escherichia coli M-8
• Escherichia coli BL21(DE3)/pN-1
• Escherichia coli BL21(DE3)/pN-2
• Escherichia coli BL21(DE3)/pN-3
• Escherichia coli BL21(DE3)/pN-4
• Escherichia coli BL21(DE3)
• Escherichia coli BW25113

Plasmids

• pQ-2
• pQ-1
• pQ-3
• pQ-4
• pN-1
• pN-2
• pN-3
• pN-4

Metabolite production

• L-alanine
  Titer
  • 80.46 g/L (Escherichia coli M-6 in Fed-Batch)
  • 7.19 g/L (Escherichia coli BL21(DE3)/pN-1 in Batch)
  • 7.81 g/L (Escherichia coli BL21(DE3)/pN-2 in Batch)
  • 6.39 g/L (Escherichia coli BL21(DE3)/pN-3 in Batch)
  • 6.52 g/L (Escherichia coli BL21(DE3)/pN-4 in Batch)
  Substrate yield
  • 1.02 g/g of sustrate (Escherichia coli M-6 in Fed-Batch)
  • 0.3905 g/g of sustrate (Escherichia coli BL21(DE3)/pN-2 in Batch)
  • 0.3595 g/g of sustrate (Escherichia coli BL21(DE3)/pN-1 in Batch)
  • 0.3195 g/g of sustrate (Escherichia coli BL21(DE3)/pN-3 in Batch)
  • 0.326 g/g of sustrate (Escherichia coli BL21(DE3)/pN-4 in Batch)