M. Lee, G. M. Smith, M. A. Eiteman & E. Altman (2004). Aerobic production of alanine by Escherichia coli aceF ldhA mutants expressing the Bacillus sphaericus alaD gene. Applied Microbiology and Biotechnology.

Strains

• Escherichia coli CGSC6162
• Escherichia coli ALS887
• Escherichia coli ALS887 pTrc99A-alaD

Plasmids

• pTrc99A-alaD

Metabolite production

• L-alanine
  Titer
  • 32.0 g/L (Escherichia coli ALS887 pTrc99A-alaD in Fed-Batch)
  Substrate yield
  • 0.63 g/g of sustrate (Escherichia coli ALS887 pTrc99A-alaD in Fed-Batch)