Yang, Hui; Zhang, Bo; Wu, Zi-Dan; Chen, Li-Feng; Pan, Jia-Yuan; Xiu, Xiao-Ling; Cai, Xue; Liu, Zhi-Qiang & Zheng, Yu-Guo. Combinatorial Metabolic Engineering of Escherichia coli for Enhanced L-Cysteine Production: Insights into Crucial Regulatory Modes and Optimization of Carbon-Sulfur Metabolism and Cofactor Availability. Journal of Agricultural and Food Chemistry. 2023, 71(36), 13409-13418.
Strains
- Escherichia coli BW01
- Escherichia coli BW02
- Escherichia coli BW03
- Escherichia coli BW04
- Escherichia coli BW05
- Escherichia coli BW06
- Escherichia coli BW07
- Escherichia coli BW08
- Escherichia coli BW08∆glyA
- Escherichia coli BW09-1
- Escherichia coli BW09-2
- Escherichia coli BW09-3
- Escherichia coli BW09-4
- Escherichia coli BW10
- Escherichia coli BW11
- Escherichia coli BW12-1
- Escherichia coli BW12-2
- Escherichia coli BW12-3
- Escherichia coli BW12-4
- Escherichia coli BW14-1
- Escherichia coli BW14-2
- Escherichia coli BW14-3
- Escherichia coli BW14-4
- Escherichia coli BW14-5
- Escherichia coli BW14-6
- Escherichia coli BW14-7
- Escherichia coli BW14-8
- Escherichia coli BW14-9
- Escherichia coli BW15-3/pED
- Escherichia coli BW02/pE
- Escherichia coli BW02/pTrc99a-cysE
- Escherichia coli BW08/pE
- Escherichia coli BW08∆glyA/pE
- Escherichia coli BW09-2/pE
- Escherichia coli BW09-2/pEB
- Escherichia coli BW11/pE
- Escherichia coli BW12-2/pE
- Escherichia coli BW12-3/pE
- Escherichia coli BW12-1/pE
- Escherichia coli BW12-4/pE
- Escherichia coli BW12-2/pserf-cysEf/pdCas/pT-null
- Escherichia coli BW13-1
- Escherichia coli BW13-1/pE
- Escherichia coli BW13-2
- Escherichia coli BW13-3
- Escherichia coli BW13-2/pE
- Escherichia coli BW13-3/pE
- Escherichia coli BW14-1/pE
- Escherichia coli BW14-5/pE
- Escherichia coli BW15-1/pE
- Escherichia coli BW15-2/pE
- Escherichia coli BW15-3/pE
- Escherichia coli BW15-2
- Escherichia coli BW15-1
- Escherichia coli BW15-3
- Escherichia coli BW25113
Plasmids
- pTrc99A
- pTrc99a-cysE
- pTrc99a-cysEf
- pTrc99a-sbp
- pTrc99a-cysUWA
- pTrc99a-cysP
- pTrc99a-cysB
- pTrc99a-cysEf-ydeD
- pTrc99a-cysEf -cysB
- pSerf-Duet
- pserf-cysEf
- pdCas
- pTarget
- pT-null
- pTarget-gpmA
- pTarget-adhE
- pTarget-pykF
Metabolite production
-
L-cysteine
-
Titer
- 0.0 g/L (Escherichia coli BW01 in Flask)
- 0.0 g/L (Escherichia coli BW02 in Flask)
- 90.9 mg/L (Escherichia coli BW02/pE in Flask)
- 423.9 mg/L (Escherichia coli BW02/pE in Flask)
- 32.8 mg/L (Escherichia coli BW02/pTrc99a-cysE in Flask)
- 598.3 mg/L (Escherichia coli BW08/pE in Flask)
- 863.5 mg/L (Escherichia coli BW08∆glyA/pE in Flask)
- 796.5 mg/L (Escherichia coli BW09-2/pE in Flask)
- 740.9 mg/L (Escherichia coli BW09-2/pEB in Flask)
- 1065.8 mg/L (Escherichia coli BW11/pE in Flask)
- 1245.8 mg/L (Escherichia coli BW12-2/pE in Flask)
- 1196.9 mg/L (Escherichia coli BW12-3/pE in Flask)
- 710.1 mg/L (Escherichia coli BW12-1/pE in Flask)
- 868.9 mg/L (Escherichia coli BW12-4/pE in Flask)
- 963.4 mg/L (Escherichia coli BW12-2/pserf-cysEf/pdCas/pT-null in Flask)
- 1348.4 mg/L (Escherichia coli BW13-1/pE in Flask)
- 1406.5 mg/L (Escherichia coli BW13-2/pE in Flask)
- 1216.7 mg/L (Escherichia coli BW13-3/pE in Flask)
- 1447.8 mg/L (Escherichia coli BW14-1/pE in Flask)
- 1607.0 mg/L (Escherichia coli BW14-5/pE in Flask)
- 1645.9 mg/L (Escherichia coli BW15-1/pE in Flask)
- 1426.6 mg/L (Escherichia coli BW15-2/pE in Flask)
- 1793.3 mg/L (Escherichia coli BW15-3/pE in Flask)
- 12.6 g/L (Escherichia coli BW15-3/pED in Fed-Batch) Volumetric productivity
- 0.21 g/L/h (Escherichia coli BW15-3/pED in Fed-Batch) Substrate yield
- 0.08 g/g of sustrate (Escherichia coli BW15-3/pED in Fed-Batch)
-
L-serine
-
Titer
- 901.3 mg/L (Escherichia coli BW08 in Flask)
-
acetate
-
Titer
- 8.1 g/L (Escherichia coli BW09-2/pE in Flask)
- 9.0 g/L (Escherichia coli BW13-3/pE in Flask)
-
(R)-lactate
-
Titer
- 5.9 g/L (Escherichia coli BW09-2/pE in Flask)
- 7.5 g/L (Escherichia coli BW13-3/pE in Flask)