Han Liu, Yu Wang, Yehua Hou & Zhimin Li (2020). Fitness of Chassis Cells and Metabolic Pathways for l‑Cysteine Overproduction in Escherichia coli. Journal of Agricultural & Food Chemistry.

Strains

Plasmids

pLH03
pLH02

Metabolite production

L-cysteine
- 190.31 mg/L (Escherichia coli JM109-pLH03 in Flask)
- 51.0 mg/L (Escherichia coli MG1655-pLH03 in Flask)
- 76.0 mg/L (Escherichia coli W3110-pLH03 in Flask)
- 7.25 g/L (Escherichia coli LH2A1M0BΔYTS-pLH03 in Fed-Batch)
- 345.74 mg/L (Escherichia coli BW25113-pLH03 in Flask)
- 438.36 mg/L (Escherichia coli LH1A-pLH03 in Flask)
- 621.14 mg/L (Escherichia coli LH2A-pLH03 in Flask)
- 286.33 mg/L (Escherichia coli LH1C-pLH03 in Flask)
- 419.21 mg/L (Escherichia coli LH2C-pLH03 in Flask)
- 459.61 mg/L (Escherichia coli LH1B-pLH03 in Flask)
- 430.95 mg/L (Escherichia coli LH2B-pLH03 in Flask)
- 634.99 mg/L (Escherichia coli LH1M-pLH03 in Flask)
- 418.12 mg/L (Escherichia coli LH2M-pLH03 in Flask)
- 393.02 mg/L (Escherichia coli LH1H-pLH03 in Flask)
- 326.24 mg/L (Escherichia coli LH2H-pLH03 in Flask)
- 106.72 mg/L (Escherichia coli LH1K-pLH03 in Flask)
- 113.74 mg/L (Escherichia coli LH2K-pLH03 in Flask)
- 370.91 mg/L (Escherichia coli LH1E-pLH03 in Flask)
- 375.87 mg/L (Escherichia coli LH2E-pLH03 in Flask)
- 414.94 mg/L (Escherichia coli LH2AC1BMH-pLH03 in Flask)
- 1026.39 mg/L (Escherichia coli LH2A1M-pLH03 in Flask)
- 1271.12 mg/L (Escherichia coli LH2A1MΔY-pLH03 in Flask)
- 1329.43 mg/L (Escherichia coli LH2A1MΔT-pLH03 in Flask)
- 1215.23 mg/L (Escherichia coli LH2A1MΔS-pLH03 in Flask)
- 1514.57 mg/L (Escherichia coli LH2A1MΔYTS-pLH03 in Flask)
- 1723.93 mg/L (Escherichia coli LH2A1M0BΔYTS-pLH03 in Flask)
- 1.97 g/L (Escherichia coli LH2A1M-pLH03 in Fed-Batch)
- 6.51 g/L (Escherichia coli LH2A1MΔYTS-pLH03 in Fed-Batch)
- 8.34 g/L (Escherichia coli LH2A1M0BΔYTS-pLH03 in Fed-Batch)