Isabel Franke, Armin Resch, Tobias Daßler, Thomas Maier & August Böck (2003). YfiK from Escherichia coli Promotes Export of O-Acetylserine and Cysteine. Journal of Bacteriology.

Strains

• Escherichia coli MC4100
• Escherichia coli MC4100Δ299
• Escherichia coli W3110
• Escherichia coli W3110Δ299
• Escherichia coli W3110 yfiK
• Escherichia coli W3110-DK
• Escherichia coli JM15
• Escherichia coli JM16
• Escherichia coli JM16/pKP290
• Escherichia coli JM16/pG2
• Escherichia coli JM16/pHCEX
• Escherichia coli JM16/pG7
• Escherichia coli W3110/pKP290
• Escherichia coli W3110/pHCEX
• Escherichia coli W3110/pG2
• Escherichia coli W3110/pG7

Plasmids

• pACYC184-LH
• pET3a
• pET3a-yfiK
• pG2
• pG7
• pGP1-2
• pHCEX
• pKO3
• pKP290
• pKP291
• pUC19
• pUC19-ΔyfiK

Metabolite production

• L-cysteine
  Titer
  • 125.0 mg/L (Escherichia coli W3110/pG7 in Flask)
  • 12.0 mg/L (Escherichia coli W3110/pG2 in Flask)
  • 0.0 mg/L (Escherichia coli W3110/pKP290 in Flask)
  • 0.0 mg/L (Escherichia coli W3110/pHCEX in Flask)
  • 0.0 mg/L (Escherichia coli JM16/pKP290 in Flask)
  • 12.0 mg/L (Escherichia coli JM16/pHCEX in Flask)
  • 145.21 mg/L (Escherichia coli JM16/pG2 in Flask)
  • 155.64 mg/L (Escherichia coli JM16/pG7 in Flask)
• O-acetyl-L-serine
  Titer
  • 0.0 mg/L (Escherichia coli W3110/pKP290 in Flask)
  • 0.0 mg/L (Escherichia coli W3110/pHCEX in Flask)
  • 30.1 mg/L (Escherichia coli W3110/pG2 in Flask)
  • 170.74 mg/L (Escherichia coli W3110/pG7 in Flask)
  • 0.0 mg/L (Escherichia coli JM16/pKP290 in Flask)
  • 0.0 mg/L (Escherichia coli JM16/pHCEX in Flask)
  • 201.78 mg/L (Escherichia coli JM16/pG2 in Flask)
  • 219.19 mg/L (Escherichia coli JM16/pG7 in Flask)