Liu, Tongle; Zhang, Kang; Zhang, Mengwei; Wang, Luyao; Wu, Yi; Cai, Bohan; Gao, Shengqi; Wu, Mengping; Wu, Wentai; Wu, Jing & Su, Lingqia. De Novo Synthesis of Lacto-N-Neotetraose in Escherichia coli through Metabolic Engineering with Glucose as the Sole Carbon Source. Journal of Agricultural and Food Chemistry. 2025, 73(22), 13736-13745.

Strains

• Escherichia coli BL21(DE3)
• Escherichia coli BL21-5
• Escherichia coli SC5

Plasmids

• pRSFDuet-1
• pCDFDuet-1
• pACYCDuet-1
• pRSFDuet-glmM-glmU-S*-Galtpm1141
• pRSFDuet-glmM-glmU-S*
• pRSFDuet-lgtA-glmM-glmU-S*