Ruxin Hao, Sumeng Wang, Xin Jin, Xiaoya Yang, Qingsheng Qi & Quanfeng Liang (2023). Dynamic and balanced regulation of the thrABC operon gene for efficient synthesis of L-threonine. Frontiers in Bioengineering and Biotechnology.
Strains
- Escherichia coli TH
- Escherichia coli 2929
- Escherichia coli 2901
- Escherichia coli 2935
- Escherichia coli 0129
- Escherichia coli 0101
- Escherichia coli 0135
- Escherichia coli 3529
- Escherichia coli 3501
- Escherichia coli 3535
- Escherichia coli Pflic-2901
- Escherichia coli P1.1-2901
- Escherichia coli P2.1-2901
- Escherichia coli DuetΔptsG
- Escherichia coli 2901ΔptsG
- Escherichia coli Pflic-2901ΔptsG
- Escherichia coli P1.1-2901ΔptsG
- Escherichia coli P2.1-2901ΔptsG
Plasmids
- pACYCDuet-1
- PA-29thrAB-29thrC
- PA-29thrAB-01thrC
- PA-29thrAB-35thrC
- PA-01thrAB-29thrC
- PA-01thrAB-01thrC
- PA-01thrAB-35thrC
- PA-35thrAB-29thrC
- PA-35thrAB-01thrC
- PA-35thrAB-35thrC
- PA-Pflic-29AB-01C
- PA-P1.1-29AB-01C
- PA-P2.1-29AB-01C
Metabolite production
-
L-threonine
-
Titer
- 40.06 g/L (Escherichia coli P2.1-2901ΔptsG in Flask)
- 26.02 g/L (Escherichia coli P2.1-2901 in Flask)
- 121.05 g/L (Escherichia coli P2.1-2901ΔptsG in Fed-Batch)
- 98.88 g/L (Escherichia coli TH in Fed-Batch) Volumetric productivity
- 1.08 g/L/h (Escherichia coli P2.1-2901 in Flask)
- 2.52 g/L/h (Escherichia coli P2.1-2901ΔptsG in Fed-Batch) Substrate yield
- 0.6 g/g of sustrate (Escherichia coli P2.1-2901ΔptsG in Fed-Batch)
- 0.49 g/g of sustrate (Escherichia coli TH in Fed-Batch)
- 2.06 g/L/h (Escherichia coli TH in Fed-Batch)