Guo, Cui; Luu, Nguyen N.T.; Adwer, Maryem M.; Hosseinzadeh, Hemen; Balan, Venkatesh; Yan, Yajun & Lin, Yuheng. Engineering Non-Natural Biosynthetic Pathways for Efficient Microbial Production of Psilocybin and Psilocin. Metabolic Engineering. 2025.

Strains

• Escherichia coli BW25113ΔtnaA
• Escherichia coli BW25113ΔtnaA (F´)
• Escherichia coli PP01
• Escherichia coli PP02
• Escherichia coli PP03

Plasmids

• pHA-GFP
• pHA-OhmK
• pHA-Arg5
• pLC-GFP
• pLC-ChuA

Metabolite production

• 4-hydroxy-L-tryptophan
  Titer
  • 149.29 mg/L (Escherichia coli PP02 in Flask)
  • 0.0 mg/L (Escherichia coli PP01 in Flask)
  • 220.72 mg/L (Escherichia coli PP02 in Flask)
  • 175.59 mg/L (Escherichia coli PP03 in Flask)
  • 761.66 mg/L (Escherichia coli PP02 in Flask)