L-homoserine charts

Titer in Flask for L-homoserine

Strain	Production
mg/L	8000.0 16000.0 24000.0 32000.0 40000.0
E. coli W-H4
E. coli W3110
E. coli HM5 (pBRmetL–pNrhtA)
E. coli W-H7
E. coli HM4
E. coli LJL3
E. coli W-8/pM1/pR1
E. coli HM4 (pBRlysCFbr)
E. coli HM4 (pBRlysC)
E. coli LJL8
E. coli LJL9
E. coli W-H7/pM1/pR1
E. coli HM4 (pBRmetL)
E. coli HM4 (pBRthrA^Fbr)
E. coli HM4 (pBRthrA)
E. coli HM4 (pBRmetL–rhtA)
E. coli LJL10
E. coli HM4 (pBRmetL–rhtA23)
E. coli LJL11
E. coli LJL12
E. coli HS4
E. coli W-9/pM1/pR1
E. coli W-10/pM1/pR1
E. coli W-H15/pM2/pR2
E. coli W-13/pM1/pR1
E. coli W-H16/pM2/pR2
E. coli W-12/pM1/pR1
E. coli W-H17/pM1/pR1
E. coli W-11/pM1/pR1
E. coli HS2
E. coli W-14/pM1/pR1
E. coli HS17
E. coli HS9
E. coli HS10
E. coli HS1
E. coli W-H15/pM1/pR1
E. coli HS6
E. coli HS12
E. coli HS3
E. coli HS5
E. coli HS7
E. coli HS16
E. coli W-H17/pM2/pR1
E. coli W-H17/pM2/pR2
E. coli HS11
E. coli W-H19/pM2/pR1
E. coli W-H18/pM2/pR1
E. coli W-H20/pM2/pR1
E. coli HS26
E. coli HS
E. coli HS22
E. coli HS18
E. coli HS19
E. coli HS8
E. coli HS15
E. coli HS25
E. coli HS13
E. coli HS20
E. coli HS14
E. coli HS23
E. coli HS21
E. coli HS24
E. coli HS27
E. coli HM4 (pBRmetL–pNrhtA)

Titer in Fed-Batch for L-homoserine

Strain	Production
mg/L	30000.0 60000.0 90000.0 120000.0 150000.0
E. coli HM3 (pBRmetL)
E. coli HM4 (pBRmetL)
E. coli HM4 (pBRmetL–pNrhtA)
E. coli LJL12
E. coli HM5 (pBRmetL–pNrhtA)
E. coli W-H18/pM2/pR1
E. coli HS27

Substrate yield in Flask for L-homoserine

Strain	Production
g/g of sustrate	0.1 0.2 0.3 0.4 0.5
E. coli W-H7
E. coli W-H7/pM1/pR1
E. coli W-9/pM1/pR1
E. coli W-H17/pM1/pR1
E. coli W-10/pM1/pR1
E. coli HM4 (pBRmetL–pNrhtA)
E. coli LJL12
E. coli W-H17/pM2/pR1
E. coli W-H17/pM2/pR2
E. coli W-H18/pM2/pR1
E. coli W-H19/pM2/pR1
E. coli W-H20/pM2/pR1

Substrate yield in Fed-Batch for L-homoserine

Strain	Production
g/g of sustrate	0.1 0.2 0.3 0.4 0.5 0.6 0.7
E. coli HM4 (pBRmetL)
E. coli HM4 (pBRmetL–pNrhtA)
E. coli HS27
E. coli W-H18/pM2/pR1

Volumetric productivity in Flask for L-homoserine

Strain	Production
mg/L/h	100 200 300 400 500 600 700 800
E. coli W-H19/pM2/pR1
E. coli W-H20/pM2/pR1
E. coli W-H18/pM2/pR1

Volumetric productivity in Fed-Batch for L-homoserine

Strain	Production
mg/L/h	400.0 800.0 1200.0 1600.0 2000.0
E. coli LJL12
E. coli HS27
E. coli W-H18/pM2/pR1