Titer in Fed-Batch for 3-methyl-2-oxobutanoate

Strain Production
mg/L
10000
20000
30000
40000
50000
60000
E. coli 050T4/pCTSDT
E. coli 050TY/pCTSDTQ487S-RBS55

Titer in Flask for 3-methyl-2-oxobutanoate

Strain Production
mg/L
5000.0
10000.0
15000.0
20000.0
25000.0
30000.0
E. coli B0016-050/pCTSDT
E. coli BL21(DE3)/pCTSDT
E. coli 050T1/pCTSDT
E. coli 050T2/pCTSDT
E. coli 050T4-1/pCTSDT
E. coli 050T4-2/pCTSDT
E. coli 050T/pCTSDT
E. coli 050T3/pCTSDT
E. coli 050T3/pCTSDT+pACYC-pntAB
E. coli 050T4/pCTSDTQ487S
E. coli 050T4/pCTSDT
E. coli 050T4/pCTSDTQ487S-RBS55
E. coli 050TY/pCTSDTQ487S-RBS55

Volumetric productivity in Fed-Batch for 3-methyl-2-oxobutanoate

Strain Production
mg/L/h
500.0
1000.0
1500.0
2000.0
2500.0
3000.0
E. coli 050T4/pCTSDT
E. coli 050TY/pCTSDTQ487S-RBS55

Biomass-specific productivity in Fed-Batch for 3-methyl-2-oxobutanoate

Strain Production
mg/L/g DCW
40.0
80.0
120.0
160.0
200.0
E. coli 050T4/pCTSDT
E. coli 050TY/pCTSDTQ487S-RBS55

Substrate yield in Fed-Batch for 3-methyl-2-oxobutanoate

Strain Production
g/g of sustrate
0.1
0.2
0.3
0.4
0.5
0.6
E. coli 050T4/pCTSDT
E. coli 050TY/pCTSDTQ487S-RBS55

Substrate yield in Flask for 3-methyl-2-oxobutanoate

Strain Production
g/g of sustrate
0.1
0.2
0.3
0.4
0.5
0.6
0.7
E. coli 050T4/pCTSDT
E. coli 050TY/pCTSDTQ487S-RBS55