Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Fed-Batch for 3-methyl-2-oxobutanoate

Modifications
Strain Maximum production ackAEc ptaEc pflBEc adhEEc frdAEc ldhAEc ilvEEc leuAEc pntAEc pntBEc ilvCEc alsSBs ilvDEc aceFEc
E. coli 050T4/pCTSDT 46,400 mg/L
E. coli 050TY/pCTSDTQ487S-RBS55 55,800 mg/L

Titer in Flask for 3-methyl-2-oxobutanoate

Modifications
Strain Maximum production ackAEc ptaEc pflBEc adhEEc frdAEc ldhAEc ilvCEc alsSBs ilvDEc ilvEEc ilvAEc leuAEc pntAEc pntBEc aceFEc
E. coli B0016-050/pCTSDT 330 mg/L
E. coli BL21(DE3)/pCTSDT 1,700 mg/L
E. coli 050T1/pCTSDT 7,360 mg/L
E. coli 050T2/pCTSDT 7,390 mg/L
E. coli 050T4-1/pCTSDT 7,560 mg/L
E. coli 050T4-2/pCTSDT 7,690 mg/L
E. coli 050T/pCTSDT 8,010 mg/L
E. coli 050T3/pCTSDT 8,440 mg/L
E. coli 050T3/pCTSDT+pACYC-pntAB 9,350 mg/L
E. coli 050T4/pCTSDTQ487S 14,880 mg/L
E. coli 050T4/pCTSDT 19,200 mg/L
E. coli 050T4/pCTSDTQ487S-RBS55 19,200 mg/L
E. coli 050TY/pCTSDTQ487S-RBS55 22,000 mg/L

Volumetric productivity in Fed-Batch for 3-methyl-2-oxobutanoate

Modifications
Strain Maximum production ackAEc ptaEc pflBEc adhEEc frdAEc ldhAEc ilvEEc leuAEc pntAEc pntBEc ilvCEc alsSBs ilvDEc aceFEc
E. coli 050T4/pCTSDT 1,780 mg/L/h
E. coli 050TY/pCTSDTQ487S-RBS55 2,140 mg/L/h

Biomass-specific productivity in Fed-Batch for 3-methyl-2-oxobutanoate

Modifications
Strain Maximum production ackAEc ptaEc pflBEc adhEEc frdAEc ldhAEc ilvEEc leuAEc pntAEc pntBEc ilvCEc alsSBs ilvDEc aceFEc
E. coli 050T4/pCTSDT 112 mg/L/g DCW
E. coli 050TY/pCTSDTQ487S-RBS55 162 mg/L/g DCW

Substrate yield in Fed-Batch for 3-methyl-2-oxobutanoate

Modifications
Strain Maximum production ackAEc ptaEc pflBEc adhEEc frdAEc ldhAEc ilvEEc leuAEc pntAEc pntBEc ilvCEc alsSBs ilvDEc aceFEc
E. coli 050T4/pCTSDT 0.41147 g/g of sustrate
E. coli 050TY/pCTSDTQ487S-RBS55 0.54437 g/g of sustrate

Substrate yield in Flask for 3-methyl-2-oxobutanoate

Modifications
Strain Maximum production ackAEc ptaEc pflBEc adhEEc frdAEc ldhAEc ilvEEc leuAEc pntAEc pntBEc ilvCEc alsSBs ilvDEc aceFEc
E. coli 050T4/pCTSDT 0.50923 g/g of sustrate
E. coli 050TY/pCTSDTQ487S-RBS55 0.6006 g/g of sustrate