Titer in Flask for L-threonine

Strain Production
mg/L
10000
20000
30000
40000
50000
E. coli MG1655
E. coli MDS42
E. coli MG-102
E. coli MDS-202
E. coli MG-103
E. coli MDS-203
E. coli MG-104
E. coli MG-105
E. coli MDS-204
E. coli MDS-205
E. coli TH07(pBRThrABC)
E. coli P2.1-2901
E. coli P2.1-2901ΔptsG

Titer in Batch for L-threonine

Strain Production
mg/L
10000
20000
30000
40000
50000
E. coli TH11C (pBRThrABC, pACYC177)
E. coli TH07 (pBRThrABC, pACYCppc)
E. coli TH07 (pBRThrABC, pACYC177)
E. coli TH07(pBRThrABC)
E. coli TH09C (pBRThrABC, pACYCaceBA)
E. coli TH19C (pBRThrABC)
E. coli TH09C (pBRThrABC, pACYC177)
E. coli TH09C (pBRThrABC)
E. coli TH20C (pBRThrABC)
E. coli TH27C (pBRThrABC)
E. coli TH27C (pBRThrABCR)
E. coli TH27C (pBRThrABCR2)
E. coli TH27C (pBRThrABCR3)
E. coli MDS-205

Titer in Fed-Batch for L-threonine

Strain Production
mg/L
30000.0
60000.0
90000.0
120000.0
150000.0
E. coli HM3 (pBRmetL)
E. coli TH27C (pBRThrABCR3)
E. coli TH28C (pBRThrABCR3)
E. coli TH
E. coli P2.1-2901ΔptsG

Substrate yield in Batch for L-threonine

Strain Production
g/g of sustrate
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
E. coli TH11C (pBRThrABC, pACYC177)
E. coli TH07 (pBRThrABC, pACYCppc)
E. coli TH07(pBRThrABC)
E. coli TH07 (pBRThrABC, pACYC177)
E. coli TH09C (pBRThrABC, pACYCaceBA)
E. coli TH19C (pBRThrABC)
E. coli TH09C (pBRThrABC, pACYC177)
E. coli TH20C (pBRThrABC)
E. coli TH27C (pBRThrABC)
E. coli TH27C (pBRThrABCR)
E. coli TH27C (pBRThrABCR2)
E. coli TH27C (pBRThrABCR3)
E. coli MDS-205
E. coli TH09C (pBRThrABC)

Substrate yield in Flask for L-threonine

Strain Production
g/g of sustrate
0.05
0.1
0.15
0.2
0.25
0.3
E. coli TH07(pBRThrABC)

Substrate yield in Fed-Batch for L-threonine

Strain Production
g/g of sustrate
500.0
1000.0
1500.0
2000.0
2500.0
3000.0
E. coli TH27C (pBRThrABCR3)
E. coli TH28C (pBRThrABCR3)
E. coli P2.1-2901ΔptsG
E. coli TH

Volumetric productivity in Flask for L-threonine

Strain Production
mg/L/h
300.0
600.0
900.0
1200.0
1500.0
E. coli P2.1-2901

Volumetric productivity in Fed-Batch for L-threonine

Strain Production
mg/L/h
500.0
1000.0
1500.0
2000.0
2500.0
3000.0
E. coli P2.1-2901ΔptsG