Titer in Flask for L-threonine

		Modifications
Strain	Maximum production	thrAEc	thrBEc	thrCEc	tdhEc	tdcCEc	rhtAEc	sstTEc	lysCEc	lysAEc	metAEc	ilvAEc	ptsGEc
E. coli MG1655	16.55 mg/L
E. coli MDS42	19.61 mg/L
E. coli MG-102	34.06 mg/L
E. coli MDS-202	36.61 mg/L
E. coli MG-103	42.25 mg/L
E. coli MDS-203	79.94 mg/L
E. coli MG-104	138.01 mg/L
E. coli MG-105	165.54 mg/L
E. coli MDS-204	228.43 mg/L
E. coli MDS-205	303.23 mg/L
E. coli TH07(pBRThrABC)	10,100 mg/L
E. coli P2.1-2901	26,020 mg/L
E. coli P2.1-2901ΔptsG	40,060 mg/L

Titer in Batch for L-threonine

		Modifications
Strain	Maximum production	thrAEc	thrBEc	thrCEc	tdhEc	tdcCEc	rhtAEc	sstTEc	lysCEc	lysAEc	metAEc	ilvAEc	iclREc	aceAEc	aceBEc	ppcEc	rhtCEc	rhtBEc
E. coli TH11C (pBRThrABC, pACYC177)	190 mg/L
E. coli TH07 (pBRThrABC, pACYCppc)	1,210 mg/L
E. coli TH07 (pBRThrABC, pACYC177)	1,460 mg/L
E. coli TH07(pBRThrABC)	1,480 mg/L
E. coli TH09C (pBRThrABC, pACYCaceBA)	1,740 mg/L
E. coli TH19C (pBRThrABC)	1,890 mg/L
E. coli TH09C (pBRThrABC, pACYC177)	1,920 mg/L
E. coli TH09C (pBRThrABC)	1,930 mg/L
E. coli TH20C (pBRThrABC)	6,390 mg/L
E. coli TH27C (pBRThrABC)	7,390 mg/L
E. coli TH27C (pBRThrABCR)	11,100 mg/L
E. coli TH27C (pBRThrABCR2)	11,590 mg/L
E. coli TH27C (pBRThrABCR3)	11,810 mg/L
E. coli MDS-205	40,100 mg/L

Titer in Fed-Batch for L-threonine

		Modifications
Strain	Maximum production	thrAEc	lysCEc	thrBEc	thrCEc	lysAEc	metAEc	ilvAEc	tdhEc	iclREc	ppcEc	tdcCEc	rhtCEc	rhtAEc	rhtBEc	acsEc	metLEc	ptsGEc
E. coli HM3 (pBRmetL)	20,620 mg/L
E. coli TH27C (pBRThrABCR3)	77,100 mg/L
E. coli TH28C (pBRThrABCR3)	82,400 mg/L
E. coli TH	98,880 mg/L
E. coli P2.1-2901ΔptsG	121,050 mg/L

Substrate yield in Batch for L-threonine

		Modifications
Strain	Maximum production	thrAEc	thrBEc	thrCEc	tdhEc	tdcCEc	rhtAEc	sstTEc	lysCEc	lysAEc	metAEc	ilvAEc	iclREc	aceAEc	aceBEc	ppcEc	rhtCEc	rhtBEc
E. coli TH11C (pBRThrABC, pACYC177)	0.028 g/g of sustrate
E. coli TH07 (pBRThrABC, pACYCppc)	0.118 g/g of sustrate
E. coli TH07(pBRThrABC)	0.137 g/g of sustrate
E. coli TH07 (pBRThrABC, pACYC177)	0.14 g/g of sustrate
E. coli TH09C (pBRThrABC, pACYCaceBA)	0.169 g/g of sustrate
E. coli TH19C (pBRThrABC)	0.183 g/g of sustrate
E. coli TH09C (pBRThrABC, pACYC177)	0.186 g/g of sustrate
E. coli TH20C (pBRThrABC)	0.213 g/g of sustrate
E. coli TH27C (pBRThrABC)	0.246 g/g of sustrate
E. coli TH27C (pBRThrABCR)	0.37 g/g of sustrate
E. coli TH27C (pBRThrABCR2)	0.386 g/g of sustrate
E. coli TH27C (pBRThrABCR3)	0.393 g/g of sustrate
E. coli MDS-205	0.401 g/g of sustrate
E. coli TH09C (pBRThrABC)	0.86 g/g of sustrate

Substrate yield in Flask for L-threonine

		Modifications
Strain	Maximum production	thrAEc	lysCEc	thrBEc	thrCEc	lysAEc	metAEc	ilvAEc	tdhEc
E. coli TH07(pBRThrABC)	0.202 g/g of sustrate

Substrate yield in Fed-Batch for L-threonine

		Modifications
Strain	Maximum production	thrAEc	lysCEc	thrBEc	thrCEc	lysAEc	metAEc	ilvAEc	tdhEc	iclREc	ppcEc	tdcCEc	rhtCEc	rhtAEc	rhtBEc	acsEc	ptsGEc
E. coli TH27C (pBRThrABCR3)	0.275 g/g of sustrate
E. coli TH28C (pBRThrABCR3)	0.294 g/g of sustrate
E. coli P2.1-2901ΔptsG	0.6 g/g of sustrate
E. coli TH	2,060 g/g of sustrate

Volumetric productivity in Flask for L-threonine

		Modifications
Strain	Maximum production	thrAEc	thrBEc	thrCEc
E. coli P2.1-2901	1,080 mg/L/h

Volumetric productivity in Fed-Batch for L-threonine

		Modifications
Strain	Maximum production	ptsGEc	thrAEc	thrBEc	thrCEc
E. coli P2.1-2901ΔptsG	2,520 mg/L/h