Titer in Flask for L-methionine

Strain Production
mg/L
1000
2000
3000
4000
5000
E. coli W3110
E. coli W3110 ΔmetJ/pTrcA*
E. coli W3110 ΔmetJ (pTrcA*H)
E. coli W3110 ΔmetJΔmetI (pTrcA*H)
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H

Titer in Fed-Batch for L-methionine

Strain Production
mg/L
2000
4000
6000
8000
10000
E. coli W3110 ΔmetJΔmetIΔlysAΔthrB/pTrcA*H
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H

Substrate yield in Batch for L-methionine

Strain Production
g/g of sustrate
0.04
0.08
0.12
0.16
0.2
E. coli W3110 ΔmetJΔmetIΔthrB/pTrcA*H
E. coli W3110 ΔmetJΔmetI (pTrcA*H)
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H
E. coli W3110 ΔmetJΔmetIΔlysAΔthrB/pTrcA*H

Substrate yield in Fed-Batch for L-methionine

Strain Production
g/g of sustrate
0.03
0.06
0.09
0.12
0.15
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H

Volumetric productivity in Fed-Batch for L-methionine

Strain Production
mg/L/h
40.0
80.0
120.0
160.0
200.0
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H