L-cysteine charts

Titer in Flask for L-cysteine

Strain	Production
mg/L	400.0 800.0 1200.0 1600.0 2000.0
E. coli BW01
E. coli BW02
E. coli W3110/pACYC184-LH
E. coli W3110/pKP290
E. coli W3110/pHCEX
E. coli JM16/pKP290
E. coli MG1655/pBluescript II SK(+)/pSTV29/pLS19
E. coli W3110/pG2
E. coli JM16/pHCEX
E. coli MG1655 (pMIV-cysE5, pACYC-yhaM1)
E. coli BW02/pTrc99a-cysE
E. coli MG1655-pLH03
E. coli ΔcysM/pDES/pCA24N
E. coli MG1655 (pMIV-cysE5)
E. coli MG1655/pCEM256I/pYdeD
E. coli W3110/pKP291
E. coli MG1655 (pMIV-cysE5, pACYC177)
E. coli W3110-pLH03
E. coli LH09
E. coli LH1K-pLH03
E. coli LH2K-pLH03
E. coli MG1655/pCEM256I/pYdeD/pLSTolC
E. coli W3110/pG7
E. coli ΔcysM/pDES/pGlpE
E. coli JM16/pG2
E. coli LH15
E. coli JM16/pG7
E. coli LH03
E. coli JM109-pLH03
E. coli LH1C-pLH03
E. coli MG1655 ΔyhaM (pMIV-cysE5)
E. coli LH2H-pLH03
E. coli BW25113-pLH03
E. coli LH1E-pLH03
E. coli LH2E-pLH03
E. coli LH1H-pLH03
E. coli LH2AC1BMH-pLH03
E. coli LH2M-pLH03
E. coli LH2C-pLH03
E. coli BW02/pE
E. coli LH2B-pLH03
E. coli LH1A-pLH03
E. coli LH1B-pLH03
E. coli LH04
E. coli LH05
E. coli LH13
E. coli LH07
E. coli BW08/pE
E. coli LH06
E. coli LH2A-pLH03
E. coli LH1M-pLH03
E. coli LH08
E. coli BW12-1/pE
E. coli BW09-2/pEB
E. coli LH14
E. coli BW09-2/pE
E. coli LH10
E. coli BW08∆glyA/pE
E. coli BW12-4/pE
E. coli MG1655ΔtnaA/pDES
E. coli BW12-2/pserf-cysEf/pdCas/pT-null
E. coli LH11
E. coli WT/pDES/pCA24N
E. coli LH2A1M-pLH03
E. coli LH12
E. coli BW11/pE
E. coli MG1655ΔtnaA/pDES/pLSTolC
E. coli BW12-3/pE
E. coli LH2A1MΔS-pLH03
E. coli BW13-3/pE
E. coli BW12-2/pE
E. coli LH2A1MΔY-pLH03
E. coli LH2A1MΔT-pLH03
E. coli BW13-1/pE
E. coli LH16
E. coli BW13-2/pE
E. coli BW15-2/pE
E. coli BW14-1/pE
E. coli LH2A1MΔYTS-pLH03
E. coli WT/pDES/pGlpE
E. coli BW14-5/pE
E. coli BW15-1/pE
E. coli LH2A1M0BΔYTS-pLH03
E. coli BW15-3/pE

Titer in Fed-Batch for L-cysteine

Strain	Production
mg/L	5000.0 10000.0 15000.0 20000.0 25000.0 30000.0
E. coli LH2A1M-pLH03
E. coli LH06
E. coli LH2A1MΔYTS-pLH03
E. coli LH16
E. coli LH2A1M0BΔYTS-pLH03
E. coli BW15-3/pED
E. coli W3110 pCys
E. coli W3110 pCysM
E. coli W3110 pCysK

Substrate yield in Flask for L-cysteine

Strain	Production
g/g of sustrate	0.01 0.02 0.03 0.04 0.05 0.06
E. coli MG1655 (pMIV-cysE5, pACYC-yhaM1)
E. coli MG1655 (pMIV-cysE5)
E. coli MG1655 (pMIV-cysE5, pACYC177)
E. coli MG1655 ΔyhaM (pMIV-cysE5)
E. coli LH03
E. coli LH04
E. coli LH05
E. coli LH06

Substrate yield in Fed-Batch for L-cysteine

Strain	Production
g/g of sustrate	0.02 0.04 0.06 0.08 0.1
E. coli W3110 pCys
E. coli W3110 pCysM
E. coli BW15-3/pED
E. coli W3110 pCysK

Volumetric productivity in Fed-Batch for L-cysteine

Strain	Production
mg/L/h	50.0 100.0 150.0 200.0 250.0 300.0
E. coli BW15-3/pED

Biomass-specific productivity in Fed-Batch for L-cysteine

Strain	Production
mg/L/g DCW	5.0 10.0 15.0 20.0 25.0 30.0
E. coli W3110 pCys
E. coli W3110 pCysM
E. coli W3110 pCysK