L-cysteine charts

Silencing

Overexpression

Feedback insensitive

Attenuation

Feed-forward insensitive

Inhibition insensitive

Unstated

Combined modifications

Titer in Flask for L-cysteine

		Modifications
Strain	Maximum production	cysE_Ec	eamA_Ec	tnaA_Ec	sdaA_Ec	serA_Ec	serB_Ec	serC_Ec	cyuA_Ec	yhaO_Ec	decR_Ec	cysM_Ec	nrdH_Ec	cysK_Ec	glpE_Ec	cysB_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	ygaP_Ec	pspE_Ec	tpiA_Ec	fbaA_Ec	zwf_Ec	talA_Ec	gapC_Ca	gapB_Bs	gapN_Sm	eamB_Ec	tolC_Ec
E. coli BW01	0 mg/L
E. coli BW02	0 mg/L
E. coli W3110/pACYC184-LH	0 mg/L
E. coli W3110/pKP290	0 mg/L
E. coli W3110/pHCEX	0 mg/L
E. coli JM16/pKP290	0 mg/L
E. coli MG1655/pBluescript II SK(+)/pSTV29/pLS19	0 mg/L
E. coli W3110/pG2	12 mg/L
E. coli JM16/pHCEX	12 mg/L
E. coli MG1655 (pMIV-cysE5, pACYC-yhaM1)	14.9 mg/L
E. coli BW02/pTrc99a-cysE	32.8 mg/L
E. coli MG1655-pLH03	51 mg/L
E. coli ΔcysM/pDES/pCA24N	63.07 mg/L
E. coli MG1655 (pMIV-cysE5)	64.6 mg/L
E. coli MG1655/pCEM256I/pYdeD	70 mg/L
E. coli W3110/pKP291	73 mg/L
E. coli MG1655 (pMIV-cysE5, pACYC177)	74.6 mg/L
E. coli W3110-pLH03	76 mg/L
E. coli LH09	85.21 mg/L
E. coli LH1K-pLH03	106.72 mg/L
E. coli LH2K-pLH03	113.74 mg/L
E. coli MG1655/pCEM256I/pYdeD/pLSTolC	119 mg/L
E. coli W3110/pG7	125 mg/L
E. coli ΔcysM/pDES/pGlpE	135.68 mg/L
E. coli JM16/pG2	145.21 mg/L
E. coli LH15	155.26 mg/L
E. coli JM16/pG7	155.64 mg/L
E. coli LH03	190.3 mg/L
E. coli JM109-pLH03	190.31 mg/L
E. coli LH1C-pLH03	286.33 mg/L
E. coli MG1655 ΔyhaM (pMIV-cysE5)	316.2 mg/L
E. coli LH2H-pLH03	326.24 mg/L
E. coli BW25113-pLH03	345.74 mg/L
E. coli LH1E-pLH03	370.91 mg/L
E. coli LH2E-pLH03	375.87 mg/L
E. coli LH1H-pLH03	393.02 mg/L
E. coli LH2AC1BMH-pLH03	414.94 mg/L
E. coli LH2M-pLH03	418.12 mg/L
E. coli LH2C-pLH03	419.21 mg/L
E. coli BW02/pE	423.9 mg/L
E. coli LH2B-pLH03	430.95 mg/L
E. coli LH1A-pLH03	438.36 mg/L
E. coli LH1B-pLH03	459.61 mg/L
E. coli LH04	492 mg/L
E. coli LH05	547.3 mg/L
E. coli LH13	550.35 mg/L
E. coli LH07	560.21 mg/L
E. coli BW08/pE	598.3 mg/L
E. coli LH06	620.9 mg/L
E. coli LH2A-pLH03	621.14 mg/L
E. coli LH1M-pLH03	634.99 mg/L
E. coli LH08	679.41 mg/L
E. coli BW12-1/pE	710.1 mg/L
E. coli BW09-2/pEB	740.9 mg/L
E. coli LH14	742.92 mg/L
E. coli BW09-2/pE	796.5 mg/L
E. coli LH10	816.36 mg/L
E. coli BW08∆glyA/pE	863.5 mg/L
E. coli BW12-4/pE	868.9 mg/L
E. coli MG1655ΔtnaA/pDES	958 mg/L
E. coli BW12-2/pserf-cysEf/pdCas/pT-null	963.4 mg/L
E. coli LH11	968.22 mg/L
E. coli WT/pDES/pCA24N	1,018.67 mg/L
E. coli LH2A1M-pLH03	1,026.39 mg/L
E. coli LH12	1,035.63 mg/L
E. coli BW11/pE	1,065.8 mg/L
E. coli MG1655ΔtnaA/pDES/pLSTolC	1,127 mg/L
E. coli BW12-3/pE	1,196.9 mg/L
E. coli LH2A1MΔS-pLH03	1,215.23 mg/L
E. coli BW13-3/pE	1,216.7 mg/L
E. coli BW12-2/pE	1,245.8 mg/L
E. coli LH2A1MΔY-pLH03	1,271.12 mg/L
E. coli LH2A1MΔT-pLH03	1,329.43 mg/L
E. coli BW13-1/pE	1,348.4 mg/L
E. coli LH16	1,359.12 mg/L
E. coli BW13-2/pE	1,406.5 mg/L
E. coli BW15-2/pE	1,426.6 mg/L
E. coli BW14-1/pE	1,447.8 mg/L
E. coli LH2A1MΔYTS-pLH03	1,514.57 mg/L
E. coli WT/pDES/pGlpE	1,529 mg/L
E. coli BW14-5/pE	1,607 mg/L
E. coli BW15-1/pE	1,645.9 mg/L
E. coli LH2A1M0BΔYTS-pLH03	1,723.93 mg/L
E. coli BW15-3/pE	1,793.3 mg/L

Titer in Fed-Batch for L-cysteine

		Modifications
Strain	Maximum production	tnaA_Ec	sdaA_Ec	serA_Ec	serB_Ec	serC_Ec	cyuA_Ec	cysM_Ec	nrdH_Ec	cysE_Ec	eamA_Ec	cysB_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	glpE_Ec	fbaA_Ec	talA_Ec	gapN_Sm	cysK_Ec
E. coli LH2A1M-pLH03	1,970 mg/L
E. coli LH06	5,100 mg/L
E. coli LH2A1MΔYTS-pLH03	6,510 mg/L
E. coli LH16	7,500 mg/L
E. coli LH2A1M0BΔYTS-pLH03	8,340 mg/L
E. coli BW15-3/pED	12,600 mg/L
E. coli W3110 pCys	16,200 mg/L
E. coli W3110 pCysM	20,400 mg/L
E. coli W3110 pCysK	23,700 mg/L

Substrate yield in Flask for L-cysteine

		Modifications
Strain	Maximum production	tnaA_Ec	sdaA_Ec	serA_Ec	serB_Ec	serC_Ec	cysE_Ec	eamA_Ec	cyuA_Ec
E. coli MG1655 (pMIV-cysE5, pACYC-yhaM1)	0.00037 g/g of sustrate
E. coli MG1655 (pMIV-cysE5)	0.00164 g/g of sustrate
E. coli MG1655 (pMIV-cysE5, pACYC177)	0.00187 g/g of sustrate
E. coli MG1655 ΔyhaM (pMIV-cysE5)	0.0081 g/g of sustrate
E. coli LH03	0.018 g/g of sustrate
E. coli LH04	0.047 g/g of sustrate
E. coli LH05	0.053 g/g of sustrate
E. coli LH06	0.06 g/g of sustrate

Substrate yield in Fed-Batch for L-cysteine

		Modifications
Strain	Maximum production	tnaA_Ec	cyuA_Ec	serA_Ec	serC_Ec	serB_Ec	sdaA_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	cysM_Ec	nrdH_Ec	glpE_Ec	fbaA_Ec	talA_Ec	gapN_Sm	cysE_Ec	eamA_Ec	cysK_Ec
E. coli W3110 pCys	0.0496 g/g of sustrate
E. coli W3110 pCysM	0.0799 g/g of sustrate
E. coli BW15-3/pED	0.08 g/g of sustrate
E. coli W3110 pCysK	0.0921 g/g of sustrate

Volumetric productivity in Fed-Batch for L-cysteine

		Modifications
Strain	Maximum production	tnaA_Ec	cyuA_Ec	serA_Ec	serC_Ec	serB_Ec	sdaA_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	cysM_Ec	nrdH_Ec	glpE_Ec	fbaA_Ec	talA_Ec	gapN_Sm	cysE_Ec	eamA_Ec
E. coli BW15-3/pED	210 mg/L/h

Biomass-specific productivity in Fed-Batch for L-cysteine

		Modifications
Strain	Maximum production	serA_Ec	cysE_Ec	eamA_Ec	cysK_Ec	cysM_Ec
E. coli W3110 pCys	16.5 mg/L/g DCW
E. coli W3110 pCysM	17.9 mg/L/g DCW
E. coli W3110 pCysK	28.1 mg/L/g DCW