Jian Wang, Li-Kun Cheng, Jing Wang, Qian Liu, Tong Shen & Ning Chen (2013). Genetic engineering of Escherichia coli to enhance production of l-tryptophan. Applied Microbiology and Biotechnology.

Strains

• Escherichia coli TRTH1013
• Escherichia coli Dmtr
• Escherichia coli Dpta
• Escherichia coli Dmtr-Y
• Escherichia coli Dpta/mtr-Y

Plasmids

• pSV-709
• pMEL03
• pMEL03-Y

Metabolite production

• acetate
  Titer
  • 0.95 g/L (Escherichia coli Dpta/mtr-Y in Fed-Batch)
• pyruvate
  Titer
  • 2.8 mM (Escherichia coli Dpta in Fed-Batch)
  • 2.5 mM (Escherichia coli Dpta/mtr-Y in Fed-Batch)
• (R)-lactate
  Titer
  • 33.7 mM (Escherichia coli Dpta in Fed-Batch)
  • 29.9 mM (Escherichia coli Dpta/mtr-Y in Fed-Batch)
• L-tryptophan
  Substrate yield
  • 18.93 % (Escherichia coli TRTH1013 in Fed-Batch)
  • 20.68 % (Escherichia coli Dpta in Fed-Batch)
  • 21.87 % (Escherichia coli Dpta/mtr-Y in Fed-Batch)
  Titer
  • 41.98 g/L (Escherichia coli TRTH1013 in Fed-Batch)
  • 46.68 g/L (Escherichia coli Dpta/mtr-Y in Fed-Batch)