Fithriani, Prayoga Suryadarma & Djumali Mangunwidjaja (2015). Metabolic Engineering of Escherichia coli Cells for Ethanol Production under Aerobic Conditions. Procedia Chemistry.

Strains

• Escherichia coli Q1 ΔopgE
• Escherichia coli JW2944 (pTadhB-pdc, pHfdh)
• Escherichia coli BW25113

Plasmids

• pUpdc
• pTadhB
• pTadhB-pdc
• pHfdh
• pUC19
• pTrc99A

Metabolite production

• pyruvate
  Titer
  • 3.83 g/L (Escherichia coli JW2944 (pTadhB-pdc, pHfdh) in Flask)
• acetate
  Titer
  • 3.67 g/L (Escherichia coli JW2944 (pTadhB-pdc, pHfdh) in Flask)
• acetaldehyde
  Titer
  • 0.01 g/L (Escherichia coli JW2944 (pTadhB-pdc, pHfdh) in Flask)
• ethanol
  Titer
  • 0.2 g/L (Escherichia coli BW25113 in Flask)
  • 1.79 g/L (Escherichia coli JW2944 (pTadhB-pdc, pHfdh) in Flask)
  • 0.4 g/L (Escherichia coli BW25113 (pTadhB-pdc) in Flask)
• (S)-lactate
  Titer
  • 0.0 g/L (Escherichia coli JW2944 (pTadhB-pdc, pHfdh) in Flask)