Du, L., Zhang, Z., Xu, Q., & Chen, N. (2019). New strategy for removing acetic acid as a by-product during L-tryptophan production. Biotechnology & Biotechnological Equipment, 33(1), 1471–1480.

Strains

• Escherichia coli T03
• Escherichia coli T10
• Escherichia coli T11
• Escherichia coli T12
• Escherichia coli T13
• Escherichia coli T14
• Escherichia coli T15
• Escherichia coli T16

Plasmids

• P1
• P2
• P3
• pTrc99A

Metabolite production

• acetate
  Titer
  • 4.2 g/L (Escherichia coli T03 in Flask)
  • 0.9 g/L (Escherichia coli T10 in Flask)
  • 1.6 g/L (Escherichia coli T11 in Flask)
  • 2.1 g/L (Escherichia coli T12 in Flask)
  • 0.7 g/L (Escherichia coli T13 in Flask)
  • 1.0 g/L (Escherichia coli T14 in Flask)
  • 1.8 g/L (Escherichia coli T15 in Flask)
  • 1.9 g/L (Escherichia coli T11 in Fed-Batch)
  • 1.4 g/L (Escherichia coli T16 in Fed-Batch)
  • 5.5 g/L (Escherichia coli T03 in Fed-Batch)
• L-tryptophan
  Titer
  • 13.3 g/L (Escherichia coli T03 in Flask)
  • 49.2 g/L (Escherichia coli T03 in Fed-Batch)
  • 10.6 g/L (Escherichia coli T10 in Flask)
  • 8.6 g/L (Escherichia coli T13 in Flask)
  • 11.6 g/L (Escherichia coli T11 in Flask)
  • 45.5 g/L (Escherichia coli T11 in Fed-Batch)
  • 9.6 g/L (Escherichia coli T14 in Flask)
  • 12.9 g/L (Escherichia coli T12 in Flask)
  • 11.1 g/L (Escherichia coli T15 in Flask)
  • 54.6 g/L (Escherichia coli T16 in Fed-Batch)