Zhen Zhang, Zichen Yu, Jinduo Wang, Yifa Yu, Lanxiao Li, Pengjie Sun, Xiaoguang Fan & Qingyang Xu (2022). Metabolic engineering of Escherichia coli for efficient production of L-5-hydroxytryptophan from glucose. Microbial Cell Factories.

Strains

• Escherichia coli HTP01
• Escherichia coli HTP02
• Escherichia coli HTP03
• Escherichia coli HTP04
• Escherichia coli HTP05
• Escherichia coli HTP06
• Escherichia coli HTP07
• Escherichia coli HTP09
• Escherichia coli HTP10
• Escherichia coli HTP11
• Escherichia coli HTP12
• Escherichia coli HTP13
• Escherichia coli HTP14
• Escherichia coli HTP08
• Escherichia coli W3110

Plasmids

• pSTV-TM
• pSTV-TM1
• pSTV-TM2
• pSTV28

Metabolite production

• 5-hydroxy-L-tryptophan
  Titer
  • 2.12 g/L (Escherichia coli HTP05 in Flask)
  • 1.02 g/L (Escherichia coli HTP04 in Flask)
  • 2.67 g/L (Escherichia coli HTP06 in Flask)
  • 0.0 g/L (Escherichia coli HTP06 in Flask)
  • 1.15 g/L (Escherichia coli HTP07 in Flask)
  • 1.71 g/L (Escherichia coli HTP08 in Flask)
  • 2.98 g/L (Escherichia coli HTP09 in Flask)
  • 8.58 g/L (Escherichia coli HTP11 in Fed-Batch)
  • 3.1 g/L (Escherichia coli HTP09 in Flask)
  • 3.2 g/L (Escherichia coli HTP10 in Flask)
  • 3.54 g/L (Escherichia coli HTP11 in Flask)
  • 2.89 g/L (Escherichia coli HTP12 in Flask)
  • 2.77 g/L (Escherichia coli HTP13 in Flask)
  • 2.18 g/L (Escherichia coli HTP14 in Flask)
  Biomass-specific yield
  • 0.108 g/g DCW (Escherichia coli HTP05 in Flask)
  • 0.058 g/L (Escherichia coli HTP04 in Flask)
  • 0.127 g/g DCW (Escherichia coli HTP06 in Flask)
  Substrate yield
  • 0.031 g/g of sustrate (Escherichia coli HTP07 in Flask)
  • 0.049 g/g of sustrate (Escherichia coli HTP08 in Flask)
  • 0.082 g/g of sustrate (Escherichia coli HTP09 in Flask)
  • 0.095 g/g of sustrate (Escherichia coli HTP11 in Fed-Batch)
  • 0.084 g/g of sustrate (Escherichia coli HTP09 in Flask)
  • 0.084 g/g of sustrate (Escherichia coli HTP10 in Flask)
  • 0.074 g/g of sustrate (Escherichia coli HTP12 in Flask)
  • 0.073 g/g of sustrate (Escherichia coli HTP13 in Flask)
  • 0.055 g/g of sustrate (Escherichia coli HTP14 in Flask)
  • 0.088 g/g of sustrate (Escherichia coli HTP11 in Flask)
  Volumetric productivity
  • 0.48 g/L/h (Escherichia coli HTP11 in Fed-Batch)
• L-tryptophan
  Titer
  • 0.0 g/L (Escherichia coli HTP06 in Flask)
  • 0.34 g/L (Escherichia coli HTP07 in Flask)
  • 0.45 g/L (Escherichia coli HTP08 in Flask)
  • 1.16 g/L (Escherichia coli HTP09 in Flask)
  • 0.5 g/L (Escherichia coli HTP11 in Fed-Batch)
  • 1.22 g/L (Escherichia coli HTP09 in Flask)
  • 1.16 g/L (Escherichia coli HTP10 in Flask)
  • 0.32 g/L (Escherichia coli HTP11 in Flask)
  • 0.21 g/L (Escherichia coli HTP12 in Flask)
  • 0.19 g/L (Escherichia coli HTP13 in Flask)
  • 0.13 g/L (Escherichia coli HTP14 in Flask)