Yuchang Luo, Qinqin Zhao, Qian Liu & Yan Feng (2019). An Artificial Biosynthetic Pathway for 2‑Amino-1,3-Propanediol Production Using Metabolically Engineered Escherichia coli. ACS Synthetic Biology.
Strains
- Escherichia coli HMS174(DE3)
- Escherichia coli HMS174ΔserB
- Escherichia coli HMS174ΔserB::rtxA-D9
- Escherichia coli HMS174ΔserB::rtxA-D9 and serB
- Escherichia coli LYC-1
- Escherichia coli LYC-2
- Escherichia coli LYC-3
- Escherichia coli LYC-4
- Escherichia coli LYC-5
- Escherichia coli HMS174(DE3)/pET-rtxA-D6
- Escherichia coli HMS174(DE3)/pET-rtxA-D7
- Escherichia coli HMS174(DE3)/pET-rtxA-D8
- Escherichia coli pET-rtxA-D9
- Escherichia coli pET-rtxA-D10
- Escherichia coli HMS174(DE3)/pACYCD-rtxA-D9
- Escherichia coli HMS174(DE3)/pCDFD-rtxA-D9
- Escherichia coli HMS174(DE3)/pETD-rtxA-D9
- Escherichia coli HMS174(DE3)/pRSFD-rtxA-D9
Plasmids
- pUC57-Up-rtxA
- pET-30a(+)
- pET-rtxA
- pET-rtxA-D1
- pET-rtxA-D2
- pET-rtxA-D3
- pET-rtxA-D4
- pET-30am-rtxA-D5
- pACYCD-rtxA-D9
- pCDFD-rtxA-D9
- pETD-rtxA-D9
- pRSFD-rtxA-D9
- pACYCD-aspAeco
- pACYCD-gdhAeco
- pACYCD-serBeco
- pACYCD-serBeco-gdhAeco
- pET-serBeco
- pET-rtxA-D6
- pET-rtxA-D7
- pET-rtxA-D8
- pET-rtxA-D9
- pET-rtxA-D10
- pCDFDuet-1
- pRSFDuet-1
- pETDuet-1
- pACYCDuet-1
Metabolite production
-
serinol
-
Titer
- 0.0 g/L (Escherichia coli pET-rtxA-D10 in Flask)
- 83.0 mg/L (Escherichia coli HMS174(DE3)/pET-rtxA-D7 in Flask)
- 264.7 mg/L (Escherichia coli pET-rtxA-D9 in Flask)
- 122.39 mg/L (Escherichia coli HMS174(DE3)/pET-rtxA-D6 in Flask)
- 180.11 mg/L (Escherichia coli HMS174(DE3)/pET-rtxA-D8 in Flask)
- 268.57 mg/L (Escherichia coli HMS174(DE3)/pETD-rtxA-D9 in Flask)
- 179.9 mg/L (Escherichia coli HMS174(DE3)/pACYCD-rtxA-D9 in Flask)
- 238.8 mg/L (Escherichia coli HMS174(DE3)/pCDFD-rtxA-D9 in Flask)
- 282.6 mg/L (Escherichia coli HMS174(DE3)/pRSFD-rtxA-D9 in Flask)
- 276.9 mg/L (Escherichia coli LYC-4 in Flask)
- 211.5 mg/L (Escherichia coli LYC-2 in Flask)
- 114.9 mg/L (Escherichia coli LYC-3 in Flask)
- 2.0 g/L (Escherichia coli LYC-4 in Flask)
- 882.65 mg/L (Escherichia coli LYC-3 in Flask)
- 940.0 mg/L (Escherichia coli LYC-2 in Flask)
- 0.0 mg/L (Escherichia coli HMS174ΔserB::rtxA-D9 in Flask)
- 3.4 g/L (Escherichia coli LYC-5 in Flask)
- 14.6 g/L (Escherichia coli LYC-5 in Fed-Batch) Substrate yield
- 0.316 mol/mol of substrate (Escherichia coli LYC-5 in Fed-Batch) Volumetric productivity
- 0.122 g/L/h (Escherichia coli LYC-5 in Fed-Batch)