Zhou, Li; Wang, Yuxi; Han, Laichuang; Wang, Qin; Liu, Haili; Cheng, Ping; Li, Ruoxuan; Guo, Xuecong & Zhou, Zhemin. Enhancement of Patchoulol Production in Escherichia coli via Multiple Engineering Strategies. Journal of Agricultural and Food Chemistry. 2021, 69(27), 7572-7580.

Strains

• Escherichia coli B0016
• Escherichia coli B0016-010
• Escherichia coli 010H
• Escherichia coli 020H
• Escherichia coli 030H
• Escherichia coli 040H
• Escherichia coli 050H
• Escherichia coli 060H
• Escherichia coli 070H
• Escherichia coli 060HA
• Escherichia coli 070HA
• Escherichia coli 070HB
• Escherichia coli 080H
• Escherichia coli 090H
• Escherichia coli PTS2
• Escherichia coli PTS1
• Escherichia coli 010H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A)
• Escherichia coli 040H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A)
• Escherichia coli 050H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A)
• Escherichia coli 060HA (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A)

Plasmids

• pMev
• pET-PTS1
• pET-PTS2
• pET-PTS2H454A
• pET-PTS2K458A
• pET-PTS2H454L
• pET-PTS2H454F
• pET-PTS2K458L
• pET-PTS2K458F
• pET-PTS2C415F
• pET-PTS2H454A/K458A
• pET-PTS2H454A/K458L
• pET-PTS2C415F/H454A
• pET-PTS2C415F/K458L
• pMevΔFPPS
• pET-FPPS-PTS2
• pET-PTS2-FPPS
• pET-FPPS-GGGS-PTS2
• pET-PTS2-GGGS-FPPS
• pET-FPPS-A(EAAA)₄A-PTS2
• pET-FPPS-(GGGGS)₃-PTS2
• pET-FPPS-(PT)₄P-PTS2
• pET-FPPS-(PA)₄-PTS2
• pET-FPPS-(PT)₄P-PTS2T7A
• pET-FPPS-(PT)₄P-PTS2C415F
• pET-FPPS-(PT)₄P-PTS2C415F/H454A

Metabolite production

• (-)-patchoulol
  Titer
  • 14.0 mg/L (Escherichia coli PTS2 in Flask)
  • 0.11 mg/L (Escherichia coli PTS1 in Flask)
  • 168.0 mg/L (Escherichia coli 010H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  • 238.0 mg/L (Escherichia coli 040H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  • 321.4 mg/L (Escherichia coli 050H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  • 338.6 mg/L (Escherichia coli 060HA (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  • 970.1 mg/L (Escherichia coli 060HA (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Fed-Batch)
  Biomass-specific yield
  • 16.04 mg/g DCW (Escherichia coli 010H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  • 25.63 mg/g DCW (Escherichia coli 050H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  • 21.4 mg/g DCW (Escherichia coli 040H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  • 48.6 mg/g DCW (Escherichia coli 060HA (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  Volumetric productivity
  • 84.6 mg/L/d (Escherichia coli 060HA (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Flask)
  • 199.0 mg/L/d (Escherichia coli 060HA (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A) in Fed-Batch)