Wu, Hongxuan; Yang, Jinhua; Shen, Peijie; Li, Qingchen; Wu, Weibin; Jiang, Xianzhang; Qin, Lina; Huang, Jianzhong; Cao, Xiao & Qi, Feng. High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered Escherichia coli. Journal of Agricultural and Food Chemistry. 2021, 69(6), 1916-1924.

Strains

• Escherichia coli MTAI-1
• Escherichia coli MTAI-2
• Escherichia coli MTAI-3
• Escherichia coli MTAI-4
• Escherichia coli MTAI-5
• Escherichia coli MIA-1
• Escherichia coli MIA-2
• Escherichia coli MIA-3
• Escherichia coli MIA-4
• Escherichia coli MIA-5
• Escherichia coli MIA-6
• Escherichia coli MGΔpgi
• Escherichia coli MGΔppc
• Escherichia coli MGΔgdhA
• Escherichia coli MGΔpgiΔgdhA
• Escherichia coli MGΔpgiΔppc
• Escherichia coli MGΔppcΔgdhA
• Escherichia coli MGΔpgiΔppcΔgdhA
• Escherichia coli MGΔadhE::icd
• Escherichia coli MGΔldhA::mdh
• Escherichia coli MG1655-trp
• Escherichia coli MG1655

Plasmids

• p6LRPW326
• pTrc99a- iaa1
• pTrc99a-iaa2
• pTrc99a-iaa3
• pTrc99a-iaa4
• pTrc99a-iaa5
• pTrc99a-iaa6
• pTrc99a-iaa9
• pTrc99a-iaa7
• pTrc99a-iaa8
• pTrc99A

Metabolite production

• (indol-3-yl)acetate
  Titer
  • 7.1 g/L (Escherichia coli MIA-6 in Flask)
  • 1.375 g/L (Escherichia coli MIA-6 in Flask)
  • 0.074 g/L (Escherichia coli MTAI-5 in Flask)
  • 0.0 g/L (Escherichia coli MTAI-1 in Flask)
  • 0.0 g/L (Escherichia coli MTAI-2 in Flask)
  • 0.03 g/L (Escherichia coli MTAI-3 in Flask)
  • 0.07 g/L (Escherichia coli MTAI-4 in Flask)
  • 0.076 g/L (Escherichia coli MIA-1 in Flask)
  • 0.26 g/L (Escherichia coli MIA-2 in Flask)
  • 0.115 g/L (Escherichia coli MIA-3 in Flask)
  • 0.76 g/L (Escherichia coli MIA-4 in Flask)
  • 1.1 g/L (Escherichia coli MIA-5 in Flask)
  • 1.38 g/L (Escherichia coli MIA-6 in Flask)
  • 0.7 g/L (Escherichia coli MG1655-trp in Flask)
  • 0.8 g/L (Escherichia coli MGΔpgi in Flask)
  • 906.0 mg/L (Escherichia coli MGΔadhE::icd in Flask)
  Biomass-specific yield
  • 1340.0 mg/g DCW (Escherichia coli MIA-6 in Flask)
• L-tryptophan
  Titer
  • 1.015 g/L (Escherichia coli MGΔpgi in Flask)
  • 1.2 g/L (Escherichia coli MGΔadhE::icd in Flask)
• (indol-3-yl)acetaldehyde
  Titer
  • 0.02 g/L (Escherichia coli MTAI-1 in Flask)
  • 0.075 g/L (Escherichia coli MTAI-2 in Flask)
  • 0.1 g/L (Escherichia coli MTAI-3 in Flask)
  • 0.12 g/L (Escherichia coli MTAI-4 in Flask)
  • 0.14 g/L (Escherichia coli MTAI-5 in Flask)
• tryptamine
  Titer
  • 0.156 g/L (Escherichia coli MTAI-1 in Flask)
  • 0.26 g/L (Escherichia coli MTAI-2 in Flask)
  • 0.31 g/L (Escherichia coli MTAI-3 in Flask)
  • 0.37 g/L (Escherichia coli MTAI-4 in Flask)
  • 0.36 g/L (Escherichia coli MTAI-5 in Flask)
• (indol-3-yl)acetamide
  Titer
  • 1.75 g/L (Escherichia coli MIA-1 in Flask)
  • 1.99 g/L (Escherichia coli MIA-2 in Flask)
  • 1.53 g/L (Escherichia coli MIA-3 in Flask)
  • 1.3 g/L (Escherichia coli MIA-4 in Flask)
  • 0.72 g/L (Escherichia coli MIA-5 in Flask)
  • 0.73 g/L (Escherichia coli MIA-6 in Flask)