Smith, Geoffrey M.; Lee, Sarah A.; Reilly, Kevin C.; Eiteman, Mark A. & Altman, Elliot. Fed-batch two-phase production of alanine by a metabolically engineered Escherichia coli. Biotechnology Letters. 2006, 28(20), 1695-1700.

Strains

• Escherichia coli YYC202
• Escherichia coli ALS929
• Escherichia coli ALS929 pTrc99A-alaD

Plasmids

• pTrc99A-alaD

Metabolite production

• acetate
  Titer
  • 10.0 g/L (Escherichia coli ALS929 pTrc99A-alaD in Batch)
  • 1.0 g/L (Escherichia coli ALS929 pTrc99A-alaD in Fed-Batch)
• L-alanine
  Titer
  • 34.0 g/L (Escherichia coli ALS929 pTrc99A-alaD in Batch)
  • 88.0 g/L (Escherichia coli ALS929 pTrc99A-alaD in Fed-Batch)
  Substrate yield
  • 0.86 g/g of sustrate (Escherichia coli ALS929 pTrc99A-alaD in Batch)
  • 1.0 g/g of sustrate (Escherichia coli ALS929 pTrc99A-alaD in Fed-Batch)
  Volumetric productivity
  • 4.0 g/L/h (Escherichia coli ALS929 pTrc99A-alaD in Fed-Batch)