Shuai Jiang, Ruirui Wang, Dehu Wang, Chunguang Zhao, Qian Ma, Heyun Wu & Xixian Xie (2023). Metabolic reprogramming and biosensor-assisted mutagenesis screening for high-level production of L-arginine in Escherichia coli. Metabolic Engineering.

Strains

• Escherichia coli MG1655
• Escherichia coli ARG1
• Escherichia coli ARG2
• Escherichia coli ARG3
• Escherichia coli ARG4
• Escherichia coli ARG5
• Escherichia coli ARG6
• Escherichia coli ERG7
• Escherichia coli ERG8
• Escherichia coli ARG9
• Escherichia coli ARG10
• Escherichia coli ARG11
• Escherichia coli ARG12
• Escherichia coli ARG13
• Escherichia coli ARG38
• Escherichia coli ARG39
• Escherichia coli ARG40
• Escherichia coli ARG41
• Escherichia coli ARG42
• Escherichia coli ARG43
• Escherichia coli ARG44
• Escherichia coli ARG19
• Escherichia coli ARG28

Metabolite production

• L-arginine
  Titer
  • 132.0 g/L (Escherichia coli ARG28 in Fed-Batch)
  • 1.9 g/L (Escherichia coli ARG2 in Flask)
  • 12.7 g/L (Escherichia coli ARG3 in Flask)
  • 13.1 g/L (Escherichia coli ARG4 in Flask)
  • 15.9 g/L (Escherichia coli ARG5 in Flask)
  • 17.7 g/L (Escherichia coli ARG6 in Flask)
  • 10.4 g/L (Escherichia coli ERG7 in Flask)
  • 21.8 g/L (Escherichia coli ARG9 in Flask)
  • 28.3 g/L (Escherichia coli ARG12 in Flask)
  • 32.7 g/L (Escherichia coli ARG19 in Flask)
  • 36.1 g/L (Escherichia coli ARG28 in Flask)
  • 33.4 g/L (Escherichia coli ARG42 in Flask)
  • 27.1 g/L (Escherichia coli ARG39 in Flask)
  • 30.9 g/L (Escherichia coli ARG41 in Flask)
  • 110.0 g/L (Escherichia coli ARG12 in Fed-Batch)
  Substrate yield
  • 0.51 g/g of sustrate (Escherichia coli ARG28 in Fed-Batch)
  • 0.14 g/g of sustrate (Escherichia coli ARG4 in Flask)
  • 0.11 g/g of sustrate (Escherichia coli ARG3 in Flask)
  • 0.33 g/g of sustrate (Escherichia coli ARG41 in Flask)
  • 0.43 g/g of sustrate (Escherichia coli ARG12 in Fed-Batch)
  Volumetric productivity
  • 2.75 g/L/h (Escherichia coli ARG28 in Fed-Batch)
  • 2.29 g/L/h (Escherichia coli ARG12 in Fed-Batch)
• acetate
  Titer
  • 5.4 g/L (Escherichia coli ARG3 in Flask)
  • 1.3 g/L (Escherichia coli ARG4 in Flask)