Caballero Cerbon, Daniel Alejandro; Widmann, Jeremias & Weuster-Botz, Dirk. Metabolic control analysis enabled the improvement of the L-cysteine production process with Escherichia coli. Applied Microbiology and Biotechnology. 2024, 108(1).

Strains

• Escherichia coli W3110
• Escherichia coli W3110 pCys
• Escherichia coli W3110 pCysK
• Escherichia coli W3110 pCysM

Plasmids

• pCys
• pCysM
• pCysK

Metabolite production

• L-cysteine
  Titer
  • 16.2 g/L (Escherichia coli W3110 pCys in Fed-Batch)
  • 20.4 g/L (Escherichia coli W3110 pCysM in Fed-Batch)
  • 23.7 g/L (Escherichia coli W3110 pCysK in Fed-Batch)
  Biomass-specific productivity
  • 16.5 mg/g DCW/h (Escherichia coli W3110 pCys in Fed-Batch)
  • 28.1 mg/g DCW/h (Escherichia coli W3110 pCysK in Fed-Batch)
  • 17.9 mg/g DCW/h (Escherichia coli W3110 pCysM in Fed-Batch)
  Substrate yield
  • 4.96 % (Escherichia coli W3110 pCys in Fed-Batch)
  • 9.21 % (Escherichia coli W3110 pCysK in Fed-Batch)
  • 7.99 % (Escherichia coli W3110 pCysM in Fed-Batch)
• N-acetyl-L-serine
  Titer
  • 29.9 g/L (Escherichia coli W3110 pCys in Fed-Batch)