Yongfei Liu, Yiran Xu, Dongqin Ding, Jianping Wen, Beiwei Zhu & Dawei Zhang (2018). Genetic engineering of Escherichia coli to improve L-phenylalanine production. BMC Biotechnology.

Strains

• Escherichia coli HD-1
• Escherichia coli Xllp02
• Escherichia coli Xllp03
• Escherichia coli Xllp04
• Escherichia coli Xllp05
• Escherichia coli Xllp06
• Escherichia coli Xllp07
• Escherichia coli Xllp08
• Escherichia coli Xllp09
• Escherichia coli Xllp10
• Escherichia coli Xllp11
• Escherichia coli Xllp23
• Escherichia coli Xllp22
• Escherichia coli Xllp21
• Escherichia coli Xllp20
• Escherichia coli Xllp19
• Escherichia coli Xllp18
• Escherichia coli Xllp17
• Escherichia coli Xllp16
• Escherichia coli Xllp15
• Escherichia coli Xllp14
• Escherichia coli Xllp13
• Escherichia coli Xllp12

Plasmids

• p-tktA
• p-ppsA
• p-talB
• p-pckA
• p-aroD
• p-ydiB
• p-aroC
• p-j23100-arod
• p-j2301-arod
• p-j23106-arod
• p-j23105-arod
• p-j23109-arod

Metabolite production

• L-phenylalanine
  Titer
  • 72.9 g/L (Escherichia coli Xllp21 in Fed-Batch)
  • 45.0 g/L (Escherichia coli HD-1 in Fed-Batch)
  • 3.06 g/L (Escherichia coli HD-1 in Flask)
  • 0.77 g/L (Escherichia coli Xllp02 in Flask)
  • 2.87 g/L (Escherichia coli Xllp03 in Flask)
  • 4.2 g/L (Escherichia coli Xllp04 in Flask)
  • 3.65 g/L (Escherichia coli Xllp05 in Flask)
  • 3.26 g/L (Escherichia coli Xllp06 in Flask)
  • 4.21 g/L (Escherichia coli Xllp07 in Flask)
  • 6.31 g/L (Escherichia coli Xllp08 in Flask)
  • 2.69 g/L (Escherichia coli Xllp09 in Flask)
  • 2.98 g/L (Escherichia coli Xllp10 in Flask)
  • 3.34 g/L (Escherichia coli Xllp11 in Flask)
  • 1.18 g/L (Escherichia coli Xllp12 in Flask)
  • 3.69 g/L (Escherichia coli Xllp13 in Flask)
  • 1.74 g/L (Escherichia coli Xllp14 in Flask)
  • 1.68 g/L (Escherichia coli Xllp15 in Flask)
  • 7.43 g/L (Escherichia coli Xllp16 in Flask)
  • 3.67 g/L (Escherichia coli Xllp17 in Flask)
  • 1.86 g/L (Escherichia coli Xllp18 in Flask)
  • 3.28 g/L (Escherichia coli Xllp19 in Flask)
  • 4.77 g/L (Escherichia coli Xllp20 in Flask)
  • 7.15 g/L (Escherichia coli Xllp21 in Flask)
  • 6.43 g/L (Escherichia coli Xllp22 in Flask)
  • 6.12 g/L (Escherichia coli Xllp23 in Flask)
  Substrate yield
  • 0.26 g/g of sustrate (Escherichia coli Xllp21 in Fed-Batch)
  • 0.186 g/g of sustrate (Escherichia coli HD-1 in Fed-Batch)
  • 0.07 g/g of sustrate (Escherichia coli HD-1 in Flask)
  • 0.09 g/g of sustrate (Escherichia coli Xllp04 in Flask)
  • 0.08 g/g of sustrate (Escherichia coli Xllp05 in Flask)
  • 0.07 g/g of sustrate (Escherichia coli Xllp06 in Flask)
  • 0.09 g/g of sustrate (Escherichia coli Xllp07 in Flask)
  • 0.12 g/g of sustrate (Escherichia coli Xllp08 in Flask)
  • 0.06 g/g of sustrate (Escherichia coli Xllp03 in Flask)
  • 0.04 g/g of sustrate (Escherichia coli Xllp02 in Flask)
  • 0.06 g/g of sustrate (Escherichia coli Xllp09 in Flask)
  • 0.07 g/g of sustrate (Escherichia coli Xllp10 in Flask)
  • 0.07 g/g of sustrate (Escherichia coli Xllp11 in Flask)
  • 0.05 g/g of sustrate (Escherichia coli Xllp12 in Flask)
  • 0.08 g/g of sustrate (Escherichia coli Xllp13 in Flask)
  • 0.05 g/g of sustrate (Escherichia coli Xllp14 in Flask)
  • 0.04 g/g of sustrate (Escherichia coli Xllp15 in Flask)
  • 0.15 g/g of sustrate (Escherichia coli Xllp16 in Flask)
  • 0.08 g/g of sustrate (Escherichia coli Xllp17 in Flask)
  • 0.04 g/g of sustrate (Escherichia coli Xllp18 in Flask)
  • 0.07 g/g of sustrate (Escherichia coli Xllp19 in Flask)
  • 0.11 g/g of sustrate (Escherichia coli Xllp20 in Flask)
  • 0.14 g/g of sustrate (Escherichia coli Xllp22 in Flask)
  • 0.13 g/g of sustrate (Escherichia coli Xllp23 in Flask)
  • 0.16 g/g of sustrate (Escherichia coli Xllp21 in Flask)