Yanan Hao, Xuewei Pan, Rufan Xing, Jiajia You, Mengkai Hu, Zhifei Liu, Xiangfei Li, Meijuan Xu & Zhiming Rao (2022). High-level production of L-valine in Escherichia coli using multi-modular engineering. Bioresource Technology.
Strains
- Escherichia coli W3110
- Escherichia coli VAL3
- Escherichia coli VAL11
- Escherichia coli VAL12
- Escherichia coli VAL13
- Escherichia coli VAL14
- Escherichia coli VAL15
- Escherichia coli VAL16
- Escherichia coli VAL17
- Escherichia coli VAL18
- Escherichia coli VAL19
- Escherichia coli VAL20
- Escherichia coli VAL21
- Escherichia coli VAL22
- Escherichia coli VAL23
- Escherichia coli VAL24
- Escherichia coli VAL25
- Escherichia coli VAL26
- Escherichia coli VAL27
- Escherichia coli VAL28
- Escherichia coli VAL29
- Escherichia coli VAL30
- Escherichia coli VAL31
- Escherichia coli VAL32
- Escherichia coli VAL33
- Escherichia coli VAL34
- Escherichia coli VAL35
- Escherichia coli VAL36
- Escherichia coli VAL37
- Escherichia coli VAL38
- Escherichia coli VAL03 ΔlacI
Plasmids
- pTrc99a-FadR
- pTrc99a-FabR
- pTrc99a-RpoS
- pTrc99a-ArcA
- pTrc99a-PdhR
- pTrc99a-FruR
- pTrc99a-IclR
- pTrc99a-Crp
- pTrc99a-Lrp
- pTrc99a-NarL
- pGRB
- pGRB-RpoS-1
- pGRB-RpoS-2
- pGRB-RpoS-3
Metabolite production
-
L-valine
-
Substrate yield
- 0.34 g/g of sustrate (Escherichia coli VAL38 in Fed-Batch)
- 0.26 g/g of sustrate (Escherichia coli VAL15 in Fed-Batch)
- 0.11 g/g of sustrate (Escherichia coli VAL12 in Flask)
- 0.15 g/g of sustrate (Escherichia coli VAL14 in Flask)
- 0.22 g/g of sustrate (Escherichia coli VAL15 in Flask)
- 0.15 g/g of sustrate (Escherichia coli VAL17 in Flask)
- 0.17 g/g of sustrate (Escherichia coli VAL18 in Flask)
- 0.045 g/g of sustrate (Escherichia coli VAL19 in Flask)
- 0.13 g/g of sustrate (Escherichia coli VAL20 in Flask)
- 0.23 g/g of sustrate (Escherichia coli VAL21 in Flask)
- 0.16 g/g of sustrate (Escherichia coli VAL22 in Flask)
- 0.14 g/g of sustrate (Escherichia coli VAL23 in Flask)
- 0.2 g/g of sustrate (Escherichia coli VAL24 in Flask)
- 0.19 g/g of sustrate (Escherichia coli VAL25 in Flask)
- 0.16 g/g of sustrate (Escherichia coli VAL26 in Flask)
- 0.33 g/g of sustrate (Escherichia coli VAL33 in Flask) Titer
- 92.0 g/L (Escherichia coli VAL38 in Fed-Batch)
- 70.0 g/L (Escherichia coli VAL15 in Fed-Batch)
- 55.0 g/L (Escherichia coli VAL15 in Fed-Batch)
- 2.9 g/L (Escherichia coli VAL3 in Flask)
- 6.85 g/L (Escherichia coli VAL12 in Flask)
- 9.35 g/L (Escherichia coli VAL14 in Flask)
- 14.1 g/L (Escherichia coli VAL15 in Flask)
- 10.9 g/L (Escherichia coli VAL16 in Flask)
- 9.95 g/L (Escherichia coli VAL17 in Flask)
- 10.9 g/L (Escherichia coli VAL18 in Flask)
- 2.85 g/L (Escherichia coli VAL19 in Flask)
- 8.6 g/L (Escherichia coli VAL20 in Flask)
- 15.0 g/L (Escherichia coli VAL21 in Flask)
- 10.3 g/L (Escherichia coli VAL22 in Flask)
- 9.15 g/L (Escherichia coli VAL23 in Flask)
- 12.95 g/L (Escherichia coli VAL24 in Flask)
- 12.1 g/L (Escherichia coli VAL25 in Flask)
- 9.95 g/L (Escherichia coli VAL26 in Flask)
- 15.9 g/L (Escherichia coli VAL27 in Flask)
- 17.4 g/L (Escherichia coli VAL30 in Flask)
- 21.25 g/L (Escherichia coli VAL33 in Flask)
- 22.35 g/L (Escherichia coli VAL38 in Flask) Volumetric productivity
- 1.46 g/L/h (Escherichia coli VAL15 in Fed-Batch)
- 1.92 g/L/h (Escherichia coli VAL38 in Fed-Batch)