Leilei Guo, Yi Cheng, Xiaoxu Tan, Hongxu Zhang, Hairong Yu, Wenjia Tian, Mingyuan Liu, Weikang Sun, Kaiyu Gao, Tianyi Jiang, Qianjin Kang, Wensi Meng, Yidong Liu, Zhaoqi Kang, Chuanjuan Lü, Chao Gao, Ping Xu & Cuiqing Ma (2025). Efficient trans-aconitic acid production using systematically metabolic engineered Escherichia coli. Synthetic and Systems Biotechnology.

Strains

• Escherichia coli W3110 (DE3)
• Escherichia coli W3110-P1
• Escherichia coli W3110-P2
• Escherichia coli W3110-P3
• Escherichia coli W3110-P4
• Escherichia coli W3110-P5
• Escherichia coli W3110-P6
• Escherichia coli W3110-TAA01
• Escherichia coli W3110-TAA02
• Escherichia coli W3110-TAA03
• Escherichia coli W3110-TAA04
• Escherichia coli W3110-TAA05
• Escherichia coli W3110-TAA06
• Escherichia coli W3110-TAA07
• Escherichia coli W3110-TAA08

Plasmids

• pETDuet-1
• pETDuet-ais-tbrB
• pETDuet-prpf-tbrB
• pETDuet-tbrA-tbrB
• pETDuet-adi1-tbrB

Metabolite production

• trans-aconitate
  Titer
  • 37.32 g/L (Escherichia coli W3110-TAA08 in Batch)
  • 3.7 g/L (Escherichia coli W3110-TAA04 in Flask)
  • 0.0 g/L (Escherichia coli W3110-TAA02 in Flask)
  • 0.362 g/L (Escherichia coli W3110-TAA01 in Flask)
  • 0.7234 g/L (Escherichia coli W3110-TAA03 in Flask)
  • 10.99 g/L (Escherichia coli W3110-TAA05 in Flask)
  • 12.92 g/L (Escherichia coli W3110-TAA06 in Flask)
  • 5.48 g/L (Escherichia coli W3110-TAA07 in Flask)
  • 15.88 g/L (Escherichia coli W3110-TAA08 in Flask)
  • 19.35 g/L (Escherichia coli W3110-TAA08 in Flask)
  Substrate yield
  • 0.76 g/g of sustrate (Escherichia coli W3110-TAA08 in Batch)
  • 0.0 g/g of sustrate (Escherichia coli W3110-TAA02 in Flask)
  • 0.0213 g/g of sustrate (Escherichia coli W3110-TAA01 in Flask)
  • 0.034 g/g of sustrate (Escherichia coli W3110-TAA03 in Flask)
  • 0.132 g/g of sustrate (Escherichia coli W3110-TAA04 in Flask)
  • 0.69 g/g of sustrate (Escherichia coli W3110-TAA07 in Flask)
  Volumetric productivity
  • 0.93 g/L/h (Escherichia coli W3110-TAA08 in Batch)
• (R)-lactate
  Titer
  • 10.75 g/L (Escherichia coli W3110 (DE3) in Flask)
• acetate
  Titer
  • 4.15 g/L (Escherichia coli W3110 (DE3) in Flask)
• succinate
  Titer
  • 1.17 g/L (Escherichia coli W3110 (DE3) in Flask)
• ethanol
  Titer
  • 2.3 g/L (Escherichia coli W3110 (DE3) in Flask)
• pyruvate
  Titer
  • 0.3 g/L (Escherichia coli W3110 (DE3) in Flask)
  • 7.45 g/L (Escherichia coli W3110-P6 in Flask)
  • 0.3 g/L (Escherichia coli W3110-P1 in Flask)
  • 5.895 g/L (Escherichia coli W3110-P2 in Flask)
  • 5.98 g/L (Escherichia coli W3110-P3 in Flask)
  • 8.0766 g/L (Escherichia coli W3110-P4 in Flask)
  • 7.95 g/L (Escherichia coli W3110-P5 in Flask)
  • 7.95 g/L (Escherichia coli W3110-P6 in Flask)
  • 8.22 g/L (Escherichia coli W3110-TAA04 in Flask)
  • 0.89 g/L (Escherichia coli W3110-TAA05 in Flask)
  • 0.1 g/L (Escherichia coli W3110-TAA05 in Flask)
  Substrate yield
  • 0.59 g/g of sustrate (Escherichia coli W3110-P6 in Flask)
  • 0.0127 g/g of sustrate (Escherichia coli W3110 (DE3) in Flask)
  • 0.0127 g/g of sustrate (Escherichia coli W3110-P1 in Flask)
  • 0.3 g/L (Escherichia coli W3110-P2 in Flask)
  • 0.3 g/g of sustrate (Escherichia coli W3110-P3 in Flask)
  • 0.432 g/g of sustrate (Escherichia coli W3110-P4 in Flask)
  • 0.432 g/g of sustrate (Escherichia coli W3110-P5 in Flask)
• formate
  Titer
  • 0.07 g/L (Escherichia coli W3110 (DE3) in Flask)