Ahn, Da-Hee; Ko, Yoo-Sung; Prabowo, Cindy Pricilia Surya & Lee, Sang Yup. Microbial production of propionic acid through a novel β-alanine route. Metabolic Engineering. 2026, 93, 219-231.

Strains

Plasmids

Metabolite production

propanoate
- 0.1729 g/L (Escherichia coli EPA1+pDtac15-At2tUS in Flask)
- 0.22 g/L (Escherichia coli EPA1+pDtac15-At2tUH in Flask)
- 0.0235 g/L (Escherichia coli EPA1+pDtac15-At2tUG in Flask)
- 0.1343 g/L (Escherichia coli EPA1+pDtac15-At2tUT in Flask)
- 0.1678 g/L (Escherichia coli EPA1+pDtac15-At2tUY in Flask)
- 0.1459 g/L (Escherichia coli EPA1+pDtac15-At2tUC in Flask)
- 0.1523 g/L (Escherichia coli EPA1+pDtac15-At2tUI in Flask)
- 0.1472 g/L (Escherichia coli EPA1+pDtac15-At2tUT_p in Flask)
- 0.1742 g/L (Escherichia coli EPA1+pDtac15-At2tUY_h in Flask)
- 0.17 g/L (Escherichia coli EPA1+pDtac15-At2tU in Flask)
- 0.24 g/L (Escherichia coli EPA1 (pDtac15-At2tUH) + pU10099-PA in Flask)
- 0.45 g/L (Escherichia coli EPA1 (pDtac15-At2tUH) + pU10099-PC in Flask)
- 0.21 g/L (Escherichia coli EPA1 (pDtac15-At2tUH) + pU10099-PB_b in Flask)
- 0.32 g/L (Escherichia coli EPA1 (pDtac15-At2tUH) + pU10099-PB_c in Flask)
- 0.54 g/L (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-PC in Flask)
- 0.7 g/L (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 0.26 g/L (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-P_cC in Flask)
- 0.08 g/L (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-P_pC in Flask)
- 0.2 g/L (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-P_rC in Flask)
- 0.03 g/L (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-P_sC in Flask)
- 5.4 g/L (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-P_pC in Fed-Batch)
- 0.61 g/L (Escherichia coli EPA2-Pzwf::Ptrc (pDtac15-At2tUH) + pU10099-P_pC in Flask)
- 0.47 g/L (Escherichia coli EPA2-Pgnd::Ptrc (pDtac15-At2tUH) + pU10099-P_pC in Flask)
- 0.53 g/L (Escherichia coli EPA2-PpntAB:Ptrc (pDtac15-At2tUH) + pU10099-P_pC in Flask)
- 0.9 g/L (Escherichia coli EPA3 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 1.25 g/L (Escherichia coli EPA4 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 14.8 g/L (Escherichia coli EPA4 (pDtac15-At2tUH) + pU10099-P_bC in Fed-Batch)
- 0.88 g/L (Escherichia coli EPA5 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 0.38 g/L (Escherichia coli EPA6 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 0.73 g/L (Escherichia coli EPA7 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 0.33 g/L (Escherichia coli EPA8 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 0.88 g/L (Escherichia coli EPA9 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 1.0 g/L (Escherichia coli EPA10 (pDtac15-At2tUH) + pU10099-P_bC in Flask)
- 0.138 g/g of sustrate (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-P_pC in Fed-Batch)
- 0.334 mol/mol of substrate (Escherichia coli EPA2 (pDtac15-At2tUH) + pU10099-P_pC in Fed-Batch)
- 0.201 g/g of sustrate (Escherichia coli EPA4 (pDtac15-At2tUH) + pU10099-P_bC in Fed-Batch)
- 0.488 mol/mol of substrate (Escherichia coli EPA4 (pDtac15-At2tUH) + pU10099-P_bC in Fed-Batch)