Nie, Yupeng; Yao, Mingjing; Jiang, Guozheng; Yang, Yi; Wang, Shenbiao; Xu, Hui; Liang, Jiayuan; Ren, Xuebing & Tian, Yanjun. Systems engineering of Escherichia coli for high-level l-alanine production. Food Bioscience. 2024, 59, 103894.

Strains

• Escherichia coli W-125
• Escherichia coli W-126
• Escherichia coli W-126-G
• Escherichia coli W-126-GA_(L)
• Escherichia coli W-126-GA_(M)
• Escherichia coli W-126-GA_(H)
• Escherichia coli W-BetT
• Escherichia coli W-P_Ala-7-BetT

Plasmids

• pTET
• pTET-gapA
• P_J23114-alaE
• P_J23105-alaE
• P_J23101-alaE
• pTET-BetT
• pTET-P_Ala-7-BetT

Metabolite production

• L-alanine
  Titer
  • 11.8 g/L (Escherichia coli W-125 in Flask)
  • 16.3 g/L (Escherichia coli W-126 in Flask)
  • 18.6 g/L (Escherichia coli W-126-G in Flask)
  • 22.8 g/L (Escherichia coli W-126-GA_(L) in Flask)
  • 24.8 g/L (Escherichia coli W-126-GA_(M) in Flask)
  • 24.9 g/L (Escherichia coli W-126-GA_(H) in Flask)
  • 80.2 g/L (Escherichia coli W-126-GA_(M) in Batch)
  • 22.3 g/L (Escherichia coli W-126-GA_(M) in Batch)
  • 102.5 g/L (Escherichia coli W-126-GA_(M) in Batch)
  • 134.5 g/L (Escherichia coli W-P_Ala-7-BetT in Batch)
  • 121.4 g/L (Escherichia coli W-BetT in Batch)
  Substrate yield
  • 0.44 g/g of sustrate (Escherichia coli W-125 in Flask)
  • 0.49 g/g of sustrate (Escherichia coli W-126 in Flask)
  • 0.57 g/g of sustrate (Escherichia coli W-126-G in Flask)
  • 0.57 g/g of sustrate (Escherichia coli W-126-GA_(M) in Batch)
  • 0.84 g/g of sustrate (Escherichia coli W-126-GA_(M) in Batch)
  • 0.87 g/g of sustrate (Escherichia coli W-BetT in Batch)
  • 0.89 g/g of sustrate (Escherichia coli W-P_Ala-7-BetT in Flask)
  Biomass-specific yield
  • 13.3 mg/g DCW (Escherichia coli W-126-G in Flask)
  • 6.0 mg/g DCW (Escherichia coli W-126-GA_(L) in Flask)
  • 3.41 mg/g DCW (Escherichia coli W-126-GA_(M) in Flask)
  • 3.26 mg/g DCW (Escherichia coli W-126-GA_(H) in Flask)
  Volumetric productivity
  • 2.23 g/L/h (Escherichia coli W-126-GA_(M) in Batch)
  • 4.27 g/L/h (Escherichia coli W-126-GA_(M) in Batch)
  • 5.6 g/L/h (Escherichia coli W-P_Ala-7-BetT in Batch)
  • 5.06 g/L/h (Escherichia coli W-BetT in Batch)
• acetate
  Titer
  • 8.62 g/L (Escherichia coli W-126-GA_(M) in Batch)
  • 1.42 g/L (Escherichia coli W-126-GA_(M) in Batch)