Chan Woo Song, Joungmin Lee, Yoo-Sung Ko & Sang Yup Lee (2015). Metabolic engineering of Escherichia coli for the production of 3-aminopropionic acid. Metabolic Engineering.

Strains

• Escherichia coli W3110
• Escherichia coli CWF4N
• Escherichia coli CWF4NA
• Escherichia coli CWF4NA2
• Escherichia coli W3110/pTac15kPC
• Escherichia coli W3110/pTac15kPE
• Escherichia coli CWF4N/pTac15kPC
• Escherichia coli CWF4NA/pTac15kPC
• Escherichia coli CWF4NA/pTac15kPAC
• Escherichia coli CWF4NA/pTac15kPC/pSynPPC1
• Escherichia coli CWF4NA/pTac15kPRA/pSynPPC1
• Escherichia coli CWF4NA/pTac15kPTA/pSynPPC1
• Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7
• Escherichia coli CWF4NA2/pTac15kPTA/pSynPPC7

Plasmids

• pEcmuloxC
• pMtrc9
• pTrc99A
• pTac15K
• pTac15kPE
• pTac15kPC
• pTac15kRAC
• pTac15kPAC
• pTac15kPRA
• pTac15kPTA
• pSynPPC1
• pSynPPC2
• pSynPPC3
• pSynPPC4
• pSynPPC5
• pSynPPC6
• pSynPPC7
• pSynPPC8
• pSynPPC9
• pSynPPC10
• pSynPPC11
• pSynPPC12

Metabolite production

• β-alanine
  Titer
  • 0.299 g/L (Escherichia coli W3110/pTac15kPC in Flask)
  • 0.0 g/L (Escherichia coli W3110/pTac15kPE in Flask)
  • 0.0 g/L (Escherichia coli CWF4N/pTac15kPC in Flask)
  • 0.855 g/L (Escherichia coli CWF4NA/pTac15kPC in Flask)
  • 0.3 g/L (Escherichia coli CWF4NA/pTac15kPAC in Flask)
  • 1.45 g/L (Escherichia coli CWF4NA/pTac15kPC/pSynPPC1 in Flask)
  • 1.63 g/L (Escherichia coli CWF4NA/pTac15kPRA/pSynPPC1 in Flask)
  • 1.66 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC1 in Flask)
  • 3.49 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC1 in Flask)
  • 3.94 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7 in Flask)
  • 20.1 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7 in Fed-Batch)
  • 32.3 g/L (Escherichia coli CWF4NA2/pTac15kPTA/pSynPPC7 in Fed-Batch)
  Substrate yield
  • 0.25 g/g of sustrate (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7 in Flask)
  • 0.135 g/g of sustrate (Escherichia coli CWF4NA2/pTac15kPTA/pSynPPC7 in Fed-Batch)
  Volumetric productivity
  • 0.457 g/L/h (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7 in Fed-Batch)
  • 0.828 g/L/h (Escherichia coli CWF4NA2/pTac15kPTA/pSynPPC7 in Fed-Batch)
• fumarate
  Titer
  • 2.85 g/L (Escherichia coli CWF4NA/pTac15kPC/pSynPPC1 in Flask)
  • 2.59 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC1 in Flask)
  • 1.0 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC1 in Flask)
  • 1.44 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7 in Flask)
  • 0.0 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7 in Fed-Batch)
• acetate
  Titer
  • 15.6 g/L (Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7 in Fed-Batch)