José-Aníbal Mora-Villalobos & An-Ping Zeng (2018). Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli. Journal of Biological Engineering.
Strains
- Escherichia coli S028
- Escherichia coli TrpD-Pl
- Escherichia coli TrpD-Gi
- Escherichia coli TrpD-Pl/pACJ23-CtAAAH-F
- Escherichia coli TrpD-PI/pACJ23-CtAAAH-LC
- Escherichia coli TrpD-Pl / pCtAAAH-LC / pCOLAJ23-TDC.2
- Escherichia coli TrpD-Pl / pCtAAAH-LC / pBAD-TDC
Plasmids
- pBbE1k-2.2
- pACPJ23
- pACPJ23-CtAAAH-F
- pACPtrc
- pACPtrc-CtAAAH-F
- pACPJ23-CtAAAH-LC
- pBAD-TDC
- pCOLADuet-TDC
Metabolite production
-
5-hydroxy-L-tryptophan
-
Titer
- 100.4 mg/L (Escherichia coli TrpD-Pl/pACJ23-CtAAAH-F in Flask)
- 962.0 mg/L (Escherichia coli TrpD-PI/pACJ23-CtAAAH-LC in Fed-Batch)
- 7.3 mg/L (Escherichia coli TrpD-Pl / pCtAAAH-LC / pCOLAJ23-TDC.2 in Fed-Batch)
- 21.3 mg/L (Escherichia coli TrpD-Pl / pCtAAAH-LC / pBAD-TDC in Fed-Batch)
-
L-tryptophan
-
Titer
- 2.7 g/L (Escherichia coli TrpD-Pl/pACJ23-CtAAAH-F in Flask)
- 23.4 g/L (Escherichia coli TrpD-PI/pACJ23-CtAAAH-LC in Fed-Batch)
- 5.34 g/L (Escherichia coli TrpD-Pl / pCtAAAH-LC / pCOLAJ23-TDC.2 in Fed-Batch)
-
serotonin
-
Titer
- 0.0 mg/L (Escherichia coli TrpD-Pl / pCtAAAH-LC / pCOLAJ23-TDC.2 in Fed-Batch)
- 0.8 mg/L (Escherichia coli TrpD-Pl / pCtAAAH-LC / pBAD-TDC in Fed-Batch)
-
tryptamine
-
Titer
- 3.03 g/L (Escherichia coli TrpD-Pl / pCtAAAH-LC / pCOLAJ23-TDC.2 in Fed-Batch)
- 1.81 g/L (Escherichia coli TrpD-Pl / pCtAAAH-LC / pBAD-TDC in Fed-Batch)