Wang, Chonglong; Yoon, Sang-Hwal; Jang, Hui-Jeong; Chung, Young-Ryun; Kim, Jae-Yean; Choi, Eui-Sung & Kim, Seon-Won. Metabolic engineering of Escherichia coli for α-farnesene production. Metabolic Engineering. 2011, 13(6), 648-655.
Strains
- Escherichia coli wFS
- Escherichia coli aFS
- Escherichia coli aFS-CT
- Escherichia coli aFS-dxs
- Escherichia coli aFS-idi
- Escherichia coli aFS-SN
- Escherichia coli ispAaFS-CT
- Escherichia coli ispAaFS-SN
- Escherichia coli ispANaFS-SN
- Escherichia coli ispALaFS-SN
- Escherichia coli DH5α
Plasmids
- pSdxs
- pSidi
- pET-30a/MdAFS1
- pUC57aFS
- pTispANaFS
- pTispALaFS
- pTispAaFS
- pTwFS
- pTaFS
- pSSN12Didi
- pUC57
- pSTV28
- pET-30a(+)
- pTrc99A
- pSNA
Metabolite production
-
(E,E)-α-farnesene
-
Biomass-specific titer
- 6.5 g/L/OD<sub>600</sub> (Escherichia coli aFS-SN in Flask) Titer
- 9.8 mg/L (Escherichia coli ispALaFS-SN in Flask)
- 1.2 mg/L (Escherichia coli wFS in Flask)
- 1.6 mg/L (Escherichia coli aFS in Flask)
- 0.9 mg/L (Escherichia coli aFS-CT in Flask)
- 1.9 mg/L (Escherichia coli aFS-dxs in Flask)
- 6.2 mg/L (Escherichia coli aFS-idi in Flask)
- 17.8 mg/L (Escherichia coli aFS-SN in Flask)
- 57.6 mg/L (Escherichia coli ispAaFS-SN in Flask)
- 2.2 mg/L (Escherichia coli ispAaFS-CT in Flask)
- 62.2 mg/L (Escherichia coli ispANaFS-SN in Flask)
- 86.8 mg/L (Escherichia coli ispALaFS-SN in Flask)
- 153.6 mg/L (Escherichia coli ispALaFS-SN in Flask)
- 380.0 mg/L (Escherichia coli ispALaFS-NA in Flask)
-
(2E,6E)-farnesol
-
Titer
- 0.0 mg/L (Escherichia coli ispAaFS-CT in Flask)
- 17.8 mg/L (Escherichia coli ispAaFS-SN in Flask)
- 22.3 mg/L (Escherichia coli ispANaFS-SN in Flask)
- 0.0 mg/L (Escherichia coli ispALaFS-SN in Flask)
-
(R)-mevalonate
-
Titer
- 16.83 mg/L (Escherichia coli ispALaFS-NA in Flask)