Nie, Mengzhen; Wang, Jingyu & Zhang, Kechun. A novel strategy for l-arginine production in engineered Escherichia coli. Microbial Cell Factories. 2023, 22(1).

Strains

• Escherichia coli N1
• Escherichia coli N2
• Escherichia coli N3
• Escherichia coli N4
• Escherichia coli N5
• Escherichia coli N6
• Escherichia coli N7
• Escherichia coli N8
• Escherichia coli N9
• Escherichia coli N10
• Escherichia coli N11
• Escherichia coli N12
• Escherichia coli BW25113

Plasmids

• pZE
• p-AGR-1^a
• p-AGR-3^a
• p-AGR-4^a
• p-ARG-2^a

Metabolite production

• L-arginine
  Titer
  • 4.0 g/L (Escherichia coli N12 in Flask)
  • 0.9 g/L (Escherichia coli N7 in Flask)
  • 0.7 g/L (Escherichia coli N6 in Flask)
  • 0.09 g/L (Escherichia coli N5 in Flask)
  • 6.7 g/L (Escherichia coli N8 in Flask)
  • 0.07 g/L (Escherichia coli BW25113 in Flask)
  • 7.6 g/L (Escherichia coli N9 in Flask)
  • 0.05 g/L (Escherichia coli N10 in Flask)
  • 1.1 g/L (Escherichia coli N11 in Flask)
  • 4.4 g/L (Escherichia coli N11 in Flask)
  Substrate yield
  • 0.69 mol/mol of substrate (Escherichia coli N8 in Flask)
  • 0.76 mol/mol of substrate (Escherichia coli N9 in Flask)
  • 0.92 mol/mol of substrate (Escherichia coli N11 in Flask)
  • 0.99 mol/mol of substrate (Escherichia coli N11 in Flask)
  Biomass-specific yield
  • 0.26 g/OD₆₀₀ (Escherichia coli N11 in Flask)
• pyruvate
  Titer
  • 11.3 g/L (Escherichia coli N12 in Flask)
  • 2.3 g/L (Escherichia coli N5 in Flask)
  • 3.0 g/L (Escherichia coli N6 in Flask)
  • 2.8 g/L (Escherichia coli N7 in Flask)
  • 2.5 g/L (Escherichia coli N8 in Flask)
  • 2.6 g/L (Escherichia coli BW25113 in Flask)
• acetate
  Titer
  • 2.7 g/L (Escherichia coli N11 in Flask)
  • 7.0 g/L (Escherichia coli N11 in Flask)