Cen, Xuecong; Liu, Yanjuan; Zhu, Fanghuan; Liu, Dehua & Chen, Zhen. Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway. Metabolic Engineering. 2022, 74, 168-177.

Strains

Plasmids

Metabolite production

1,5-pentanediol
- 3.86 g/L (Escherichia coli S04 in Flask)
- 4.58 g/L (Escherichia coli S05 in Flask)
- 3.23 g/L (Escherichia coli S06 in Flask)
- 3.88 g/L (Escherichia coli S07 in Flask)
- 3.18 g/L (Escherichia coli S08 in Flask)
- 3.16 g/L (Escherichia coli S09 in Flask)
- 1.0 g/L (Escherichia coli AC01 in Flask)
- 1.52 g/L (Escherichia coli AC01 in Flask)
- 1.86 g/L (Escherichia coli AC02 in Flask)
- 1.88 g/L (Escherichia coli AC03 in Flask)
- 2.62 g/L (Escherichia coli AC04 in Flask)
- 9.25 g/L (Escherichia coli AC04 in Fed-Batch)
- 0.16 g/g of sustrate (Escherichia coli AC04 in Fed-Batch)
- 0.28 mol/mol of substrate (Escherichia coli AC04 in Fed-Batch)
5-hydroxypentanoate
- 0.64 g/L (Escherichia coli S10 in Flask)
- 0.37 g/L (Escherichia coli S12 in Flask)
- 0.61 g/L (Escherichia coli S13 in Flask)
- 3.72 g/L (Escherichia coli AC04 in Fed-Batch)
5-aminopentanoate
- 0.2 g/L (Escherichia coli S10 in Flask)
- 0.03 g/L (Escherichia coli S11 in Flask)
- 1.72 g/L (Escherichia coli S12 in Flask)
- 0.26 g/L (Escherichia coli S13 in Flask)
- 0.0 g/L (Escherichia coli AC01 in Flask)
glutarate
- 0.33 g/L (Escherichia coli S11 in Flask)
- 0.1 g/L (Escherichia coli S10 in Flask)
- 0.56 g/L (Escherichia coli S13 in Flask)
- 0.49 g/L (Escherichia coli S12 in Flask)
- 0.05 g/L (Escherichia coli AC01 in Flask)
- 0.1 g/L (Escherichia coli AC02 in Flask)
cadaverine
- 3.54 g/L (Escherichia coli AC01 in Flask)
- 0.81 g/L (Escherichia coli AC01 in Flask)
- 1.28 g/L (Escherichia coli AC02 in Flask)
- 1.56 g/L (Escherichia coli AC04 in Flask)
- 2.23 g/L (Escherichia coli AC04 in Fed-Batch)
- 0.44 g/L (Escherichia coli AC04 in Fed-Batch)
L-lysine
- 0.0 g/L (Escherichia coli AC01 in Flask)
acetate
- 5.3 g/L (Escherichia coli AC04 in Fed-Batch)