Sheng, Qi; He, Shengyang; Liang, Guangjie; Meng, Gang; Zhao, Chunguang; Wei, Aiying; Gou, Lining; Liu, Jia; Li, Xiaomin; Wu, Jing & Liu, Liming. Improved arginine production in Escherichia coli by harnessing the intracellular citrulline. Metabolic Engineering. 2025.

Strains

Plasmids

Metabolite production

L-arginine
- 108.33 g/L (Escherichia coli Arg10 in Fed-Batch)
- 34.64 g/L (Escherichia coli Arg0 in Fed-Batch)
- 41.89 g/L (Escherichia coli Arg2 in Fed-Batch)
- 52.83 g/L (Escherichia coli Arg4 in Fed-Batch)
- 78.11 g/L (Escherichia coli Arg4 in Fed-Batch)
- 64.02 g/L (Escherichia coli Arg4 in Fed-Batch)
- 58.46 g/L (Escherichia coli Arg4 in Fed-Batch)
- 18.12 g/L (Escherichia coli Arg4-1 in Flask)
- 15.73 g/L (Escherichia coli Arg4-2 in Flask)
- 17.66 g/L (Escherichia coli Arg4-3 in Flask)
- 16.24 g/L (Escherichia coli Arg4-4 in Flask)
- 16.09 g/L (Escherichia coli Arg4-5 in Flask)
- 15.31 g/L (Escherichia coli Arg4-6 in Flask)
- 15.76 g/L (Escherichia coli Arg4-7 in Flask)
- 15.25 g/L (Escherichia coli Arg4-8 in Flask)
- 20.15 g/L (Escherichia coli Arg5 in Flask)
- 23.08 g/L (Escherichia coli Arg6 in Flask)
- 26.89 g/L (Escherichia coli Arg7 in Flask)
- 30.01 g/L (Escherichia coli Arg8 in Flask)
- 34.08 g/L (Escherichia coli Arg9 in Flask)
- 36.14 g/L (Escherichia coli Arg10 in Flask)
- 71.27 g/L (Escherichia coli Arg6 in Fed-Batch)
- 80.51 g/L (Escherichia coli Arg8 in Fed-Batch)
- 86.24 g/L (Escherichia coli Arg10 in Fed-Batch)
- 4.75 g/L (Escherichia coli Arg6 in Fed-Batch)
- 0.54 g/g of sustrate (Escherichia coli Arg10 in Fed-Batch)
- 0.21 g/g of sustrate (Escherichia coli Arg0 in Fed-Batch)
- 0.25 g/g of sustrate (Escherichia coli Arg2 in Fed-Batch)
- 0.3 g/g of sustrate (Escherichia coli Arg4 in Fed-Batch)
- 0.38 g/g of sustrate (Escherichia coli Arg6 in Fed-Batch)
- 0.42 g/g of sustrate (Escherichia coli Arg8 in Fed-Batch)
- 0.45 g/g of sustrate (Escherichia coli Arg10 in Fed-Batch)
- 2.26 g/L/h (Escherichia coli Arg10 in Fed-Batch)
- 0.72 g/L/h (Escherichia coli Arg0 in Fed-Batch)
- 0.87 g/L/h (Escherichia coli Arg2 in Fed-Batch)
- 1.1 g/L/h (Escherichia coli Arg4 in Fed-Batch)
- 1.48 g/L/h (Escherichia coli Arg6 in Fed-Batch)
- 1.68 g/L/h (Escherichia coli Arg8 in Fed-Batch)
- 1.8 g/L/h (Escherichia coli Arg10 in Fed-Batch)
L-citrulline
- 9.91 g/L (Escherichia coli Arg4 in Fed-Batch)
- 6.47 g/L (Escherichia coli Arg2 in Fed-Batch)
- 2.32 g/L (Escherichia coli Arg0 in Fed-Batch)
- 3.31 g/L (Escherichia coli Arg4 in Fed-Batch)
- 4.98 g/L (Escherichia coli Arg4 in Fed-Batch)
- 6.85 g/L (Escherichia coli Arg4 in Fed-Batch)
- 1.15 g/L (Escherichia coli Arg10 in Fed-Batch)
- 2.33 g/L (Escherichia coli Arg8 in Fed-Batch)